UBE2D4

UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.

UBE2D4 Related Areas

Enzyme>>Protease & Regulator>>Other Proteases>> UBE2D4

Neuroscience>>Neurodegenerative Disease>>Ubiquitin-proteasome pathway>>UBE2D4

UBE2D4 Alternative Names

HBUCE1 [Homo sapiens]

Human UBE2D4 Protein General Information

 

• Protein names: Ubiquitin-conjugating enzyme E2 D4
  Short name=UBE2D4
• Sequence length: 147 AA.
• Pathway: Protein modification; protein ubiquitination.
• Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
• Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

General information above from UniProt

Function for UBE2D4 Protein

UniProtKB:

UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2D4 is in vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.