UBE2D1 / UBCH5 Protein

Ubiquitin-conjugating enzyme E2D 1

UBE2D1 / UBCH5 Products

UBE2D1 / UBCH5 Protein, Recombinant

Molecule	Species	Description	Cat. No
UBE2D1/UBCH5	Human	UBE2D1/UBCH5 Protein, Recombinant	11432-H07E

UBE2D1 / UBCH5 cDNA Clone

Molecule	Species	Description	Cat. No
UBE2D1/UBCH5	Human	Human UBE2D1/UBCH5 cDNA Clone / ORF Clone	HG11432-M

UBE2D1 / UBCH5 Alternative Names

UBE2D1, UBCH5, E2(17)KB1, SFT, UBC4/5, UBCH5A [Homo sapiens]

Ube2d1, UBCH5, MGC28550 [Mus musculus]

UBE2D1 / UBCH5 Background

UBE2D1 (Ubiquitin-conjugating enzyme E2D 1), also known as UBCH5, or UBCH5A, is a member of the ubiquitin-conjugating enzyme UBE2D family. UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system. Analysis of the IDOL-UBE2D1 interface also defined the stereochemical basis for the selectivity of IDOL for UBE2Ds over other E2 ligases. The IDOL-UBE2D complex as an important determinant of LDLR activity, and provide insight into molecular mechanisms underlying the regulation of cholesterol uptake. UBCH5 regulates Zipper-interacting protein kinase (ZIPK) accumulation in PML-NBs by interacting with ZIPK and stimulating its ubiquitination. Cadmium (Cd) markedly decreased the expression of UBE2D1, UBE2D2, UBE2D3 and UBE2D4 prior to the appearance of cytotoxicity in the NRK-52E cells. UBE2D family members may be one of the critical targets of renal toxicity caused by Cd. UBCH5A is involved in the iron-dependent ubiquitination of the hypoxia-inducible factor (HIF) by the von Hippel-Lindau tumor suppressor (pVHL) E3 ligase complex.

UBE2D1 / UBCH5 Related Studies

1. Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74.
2. Tokumoto M, et al. (2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200.
3. Ohbayashi N, et al. (2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12.
4. Gehrke SG, et al. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.