UBE2A / HHR6A Protein (His Tag)

Ubiquitin-conjugating enzyme E2A P Protein Datasheet

UBE2A / HHR6A Protein Product Information

• Synonym: HHR6A, RAD6A, UBC2
• Protein Construction: A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152 ) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.Coli

UBE2A / HHR6A Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human UBE2A consisting of 167 amino acids and has a calculated molecular mass of 19.2KDa. It migrates as an approximately 18.5KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, 20%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: UBE2A / HHR6A protein

UBE2A / HHR6A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

UBE2A / HHR6A Protein Description

Ubiquitin-conjugating enzyme E2 A( also known as hHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. hHR6A is described as a CDK2 substrate. hHR6A is phosphorylated in vitro by CDK-1 and CDK-2 on Ser120, a residue conserved in all hHR6A homologues, resulting in a 4-fold increase in its ubiquitin-conjugating activity. In vivo, hHR6A phosphorylation peaks during the G2/M phase of cell cycle transition, with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished hHR6A activity, while mutation to aspartate to mimic phosphorylated serine increased hHR6A activity 3-fold. A mutation of HHR6A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.

References

1. Nascimento RM. et al., 2006, Am J Hum Genet. 79 (3): 549-55.
2. Koken MH. et al., 1992, Genomics. 12 (3): 447-53.
3. Sarcevic B. et al., 2002, EMBO J. 21 (8): 2009-18.