UBCH8 / UBE2L6 Protein (His Tag)

Ubiquitin-conjugating enzyme E2L 6 Protein Datasheet

UBCH8 / UBE2L6 Protein Product Information

• Synonym: RIG-B, UBCH8
• Protein Construction: A DNA sequence encoding the human UBE2L6 isoform 1(O14933-1) (Met 1-Ser 153) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

UBCH8 / UBE2L6 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human UBE2L6 consisting of 168 amino acids and has a calculated molecular mass of 19.6KDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from sterile 20mM Tris, 0.1% Brij35; pH8.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: UBCH8 / UBE2L6 protein

UBCH8 / UBE2L6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

UBCH8 / UBE2L6 Protein Description

UBCH8, also known as UBE2L6, is a member of the ubiquitin-conjugating enzyme family. UBCH8 functions in ubiquitination. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBCH8 is a member of the E2 ubiquitin-conjugating enzyme family. It is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. UBCH8 catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. It functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.

References

1. Moynihan TP. et al., 1999, J Biol Chem. 274 (43): 30963-8.
2. Ardley HC. et al., 2000, Cytogenet Cell Genet. 89 (1-2): 137-40.
3. Zhao C. et al., 2004, Proc Natl Acad. 101 (20): 7578-82.
4. Tripathi MK. et al., 2005, Biochem Biophys Res Commun. 328 (1): 43-8.