Human UBASH3B Gene cDNA Clone / ORF Clone

UBASH3B cDNA Clone Datasheet

UBASH3B cDNA Clone Product Information

• Gene Bank Ref.ID: BC007541
• cDNA Size: 1950
• Gene Synonym: KIAA1959, MGC15437, STS-1, STS1, TULA-2, p70,UBASH3B
• Source: Human

UBASH3B cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ubiquitin associated and SH3 domain containing, B DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

UBASH3B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UBASH3B cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UBASH3B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCAGT ACGGCCACCC CAGTCCGCTC GGCATGGCTG CGAGAGAGGA GCTGTACAGC
61       AAAGTCACCC CCCGGAGGAA CCGCCAACAG CGCCCCGGCA CCATCAAGCA TGGATCGGCG
121      CTGGACGTGC TCCTCTCCAT GGGGTTCCCC AGAGCCCGCG CACAAAAAGC CTTGGCATCC
181      ACGGGAGGAA GAAGTGTTCA GGCAGCATGT GACTGGTTAT TCTCCCATGT CGGTGACCCC
241      TTCCTGGATG ACCCCCTGCC CCGGGAGTAC GTCCTCTACC TCCGTCCCAC CGGCCCCTTA
301      GCACAGAAGC TTTCCGACTT TTGGCAGCAG TCGAAGCAGA TCTGCGGGAA GAACAAGGCA
361      CACAACATCT TCCCCCACAT CACACTCTGC CAGTTCTTTA TGTGCGAGGA CAGCAAGGTG
421      GATGCCCTGG GGGAAGCCCT GCAGACCACG GTCAGTCGCT GGAAATGTAA GTTCTCGGCC
481      CCGCTGCCCC TGGAGCTCTA TACGTCGTCC AACTTCATCG GCCTCTTTGT AAAGGAAGAC
541      AGTGCGGAGG TCCTCAAGAA GTTTGCTGCT GACTTTGCTG CAGAGGCTGC ATCCAAAACC
601      GAAGTGCATG TGGAACCTCA TAAGAAGCAG CTACATGTGA CCCTGGCTTA CCACTTCCAA
661      GCCAGCCACC TACCCACCCT AGAGAAACTG GCCCAGAACA TTGACGTCAA GCTAGGGTGT
721      GACTGGGTGG CTACCATATT TTCTCGGGAT ATCCGATTTG CTAACCATGA GACATTACAG
781      GTCATCTACC CCTATACCCC ACAAAATGAC GATGAGCTGG AGCTGGTCCC CGGGGACTTC
841      ATCTTCATGT CTCCAATGGA GCAGACCAGC ACCAGCGAGG GTTGGATCTA TGGCACGTCC
901      TTAACCACCG GCTGCTCTGG ACTCCTGCCT GAGAATTACA TTACCAAGGC TGATGAATGC
961      AGCACCTGGA TATTTCATGG TTCTTATTCA ATCTTAAATA CATCGTCATC CAACTCTCTC
1021     ACGTTTGGGG ATGGAGTATT GGAGAGGCGG CCTTATGAGG ACCAGGGGCT CGGGGAGACG
1081     ACTCCTCTTA CTATCATCTG CCAGCCCATG CAGCCGCTGA GGGTCAACAG CCAGCCCGGC
1141     CCCCAGAAGC GATGCCTTTT TGTGTGTCGG CATGGTGAGA GGATGGATGT TGTGTTTGGG
1201     AAGTACTGGC TGTCCCAGTG CTTCGATGCC AAAGGCCGCT ACATACGCAC CAACCTGAAC
1261     ATGCCTCATA GTTTACCTCA GCGGAGTGGT GGTTTCCGAG ATTACGAGAA AGATGCTCCC
1321     ATCACTGTGT TTGGATGCAT GCAAGCAAGA CTAGTGGGTG AAGCCTTATT AGAGAGCAAT
1381     ACCATTATCG ATCATGTCTA TTGCTCCCCG TCCCTTCGCT GCGTTCAGAC TGCACACAAT
1441     ATCTTGAAAG GTTTACAACA AGAAAATCAC TTGAAGATCC GTGTAGAGCC CGGCTTATTT
1501     GAGTGGACAA AATGGGTTGC TGGGAGCACA TTACCTGCAT GGATACCTCC ATCAGAGTTA
1561     GCTGCAGCCA ACCTGAGTGT TGATACAACC TACAGACCTC ACATTCCAAT CAGCAAATTA
1621     GTTGTTTCAG AATCCTATGA TACTTATATC AGTAGAAGTT TCCAAGTAAC AAAAGAAATA
1681     ATAAGTGAAT GTAAAAGTAA AGGAAATAAC ATCCTGATTG TGGCCCACGC ATCTTCCCTT
1741     GAAGCGTGTA CCTGCCAACT TCAGGGCCTG TCACCTCAGA ACTCCAAGGA CTTCGTACAA
1801     ATGGTCCGAA AGATCCCATA TCTGGGATTT TGTTCCTGTG AAGAATTAGG AGAAACTGGA
1861     ATATGGCAGC TGACAGATCC ACCAATCCTT CCTCTTACCC ATGGACCAAC TGGGGGCTTC
1921     AACTGGAGAG AGACCTTGCT TCAAGAATAA

 

Quality Control:cDNA sequencing results is available upon request.