Human UBA6 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12240-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

UBA6 cDNA Clone Datasheet

UBA6 cDNA Clone Product Information

Gene Bank Ref.ID:	BC031637
cDNA Size:	1170
Gene Synonym:	E1-L2; MOP-4; UBE1L2；UBA6
Source:	Human

UBA6 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ubiquitin-like modifier activating enzyme 6 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1633 A/G not causing the amino acid variation.

UBA6 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UBA6 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UBA6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG
61       GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT
121      GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC
181      AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT
241      GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT
301      CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA
361      GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT
421      CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT
481      GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA
541      ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT
601      GATGAATTTG AAGTTTTAGA TACAACAGGA GAGGAACCAA AAGAAATTTT CATTTCAAAC
661      ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG
721      ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA
781      CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA
841      CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA
901      TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG
961      GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT
1021     CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT
1081     ATAAGTGAAA CCTTGGAAGA GAAGGTGACT ATTGAAATTT ATGGCTGTCC GAATATTTGT
1141     TTGTTAATAC ATAAGTGTTC TGTATATTAG

Quality Control:cDNA sequencing results is available upon request.