Human UACA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13514-G	Cloning vector ($395) pCMV / hygro ($645) pCMV / hygro - HA ($645) pCMV / hygro - His ($645) pCMV / hygro - Myc ($645) pCMV / hygro - FLAG ($645)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

UACA cDNA Clone Datasheet

UACA cDNA Clone Product Information

Gene Bank Ref.ID:	NM_018003.2
cDNA Size:	4251
Gene Synonym:	NUCLING; FLJ10128; KIAA1561; MGC141967; MGC141969; UACA
Source:	Human

UACA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens uveal autoantigen with coiled-coil domains and ankyrin repeats DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 2955 C/Tnot causing the amino acid variation.

UACA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UACA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UACA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGAGCC TCAAGTCCCG CCTGAGGAGG CAGGACGTGC CCGGCCCCGC GTCGTCTGGC
61       GCCGCCGCCG CCAGCGCGCA TGCAGCAGAT TGGAATAAAT ATGATGACCG ATTGATGAAA
121      GCAGCAGAAA GGGGGGATGT AGAAAAAGTG ACCTCAATCC TTGCTAAAAA GGGGGTCAAT
181      CCAGGCAAAC TAGATGTGGA AGGCAGATCT GTCTTCCATG TTGTGACCTC AAAGGGGAAT
241      CTTGAGTGTT TGAATGCCAT CCTTATACAT GGAGTTGATA TTACAACCAG TGACACTGCA
301      GGGAGAAATG CTCTTCACCT GGCTGCTAAG TATGGACATG CATTGTGCCT ACAAAAACTT
361      CTACAGTACA ATTGTCCCAC TGAGCATGCA GACCTGCAGG GAAGAACTGC ACTTCACGAT
421      GCCGCAATGG CAGATTGTCC TTCTAGCATA CAGCTGCTTT GTGACCATGG GGCCTCTGTG
481      AATGCCAAAG ATGTAGACGG GCGGACACCA CTTGTTCTGG CTACTCAGAT GAGTAGGCCA
541      ACAATATGTC AACTGCTGAT AGATAGAGGA GCGGATGTTA ATTCCAGAGA CAAACAAAAC
601      AGAACTGCCC TCATGCTAGG TTGCGAATAT GGTTGCAGAG ATGCAGTAGA AGTCTTAATT
661      AAAAATGGTG CTGATATAAG CTTGCTGGAT GCGCTTGGCC ATGATAGTTC TTACTATGCA
721      AGAATTGGTG ACAATCTGGA CATTCTAACC TTGTTGAAGA CTGCATCGGA AAATACCAAC
781      AAAGGGAGAG AACTTTGGAA GAAAGGGCCA TCTTTGCAAC AGCGAAATTT GACACACATG
841      CAAGATGAAG TAAATGTGAA GTCACATCAG AGGGAGCATC AAAATATTCA GGATTTGGAG
901      ATTGAAAATG AAGATTTGAA AGAGAGGTTG AGAAAAATTC AGCAAGAACA AAGAATACTT
961      TTGGATAAAG TCAATGGTTT ACAGTTACAG CTGAATGAGG AAGTTATGGT TGCTGATGAT
1021     CTGGAAAGCG AGAGAGAAAA GCTGAAGTCC CTTTTGGCAG CTAAAGAAAA GCAACATGAA
1081     GAAAGCTTAA GGACTATTGA GGCTCTGAAA AATAGATTTA AATATTTTGA GAGTGATCAT
1141     TTAGGATCAG GAAGTCATTT CAGTAACCGA AAAGAAGATA TGCTTCTTAA ACAAGGTCAG
1201     ATGTATATGG CAGACTCACA GTGTACTTCC CCAGGTATAC CAGCCCATAT GCAAAGCAGA
1261     TCTATGTTAA GACCTCTGGA ACTATCTTTA CCCAGTCAAA CGTCATACTC TGAAAATGAA
1321     ATTTTAAAGA AAGAGTTAGA AGCAATGCGA ACTTTCTGTG AGTCAGCAAA ACAAGACCGA
1381     CTGAAGCTCC AAAATGAACT GGCACACAAA GTGGCAGAAT GCAAAGCTTT AGCATTAGAA
1441     TGTGAAAGGG TCAAGGAGGA TTCAGATGAA CAGATAAAGC AATTAGAAGA TGCATTAAAA
1501     GATGTGCAGA AGAGGATGTA TGAGTCAGAA GGTAAAGTTA AACAAATGCA GACCCATTTT
1561     CTTGCCCTTA AAGAACACTT AACAAGTGAA GCAGCCTCAG GGAATCACAG ACTAACCGAG
1621     GAACTGAAGG ATCAGTTGAA AGACTTGAAA GTAAAATATG AAGGTGCTTC AGCAGAAGTG
1681     GGGAAATTAA GAAACCAAAT CAAACAAAAT GAGATGATAG TAGAAGAGTT TAAGAGGGAT
1741     GAAGGCAAGC TGATAGAAGA AAATAAGCGA TTACAGAAGG AACTTAGTAT GTGTGAAATG
1801     GAGCGAGAGA AGAAAGGAAG AAAGGTCACA GAGATGGAAG GCCAGGCAAA AGAATTGTCA
1861     GCGAAGTTGG CCCTTTCCAT TCCAGCTGAA AAATTTGAAA ACATGAAGAG CTCATTATCA
1921     AATGAAGTGA ATGAGAAAGC AAAAAAATTA GTAGAAATGG AAAGAGAACA TGAAAAATCA
1981     CTTAGTGAAA TTAGACAGTT AAAGAGAGAA CTTGAGAATG TTAAGGCCAA GCTTGCTCAG
2041     CACGTCAAAC CAGAGGAACA TGAACAGGTT AAGAGCAGAT TAGAACAGAA ATCAGGAGAA
2101     CTTGGGAAGA AGATCACTGA GTTAACATTG AAAAATCAGA CACTACAAAA GGAAATTGAA
2161     AAAGTTTATT TGGATAATAA GCTCCTCAAG GAGCAAGCAC ATAACTTAAC AATTGAAATG
2221     AAAAATCATT ATGTTCCTTT AAAAGTAAGT GAAGACATGA AAAAGTCACA TGATGCAATT
2281     ATTGATGATC TTAATAGAAA GCTTTTAGAT GTAACACAAA AATATACAGA AAAGAAGTTG
2341     GAAATGGAGA AATTGCTACT GGAAAATGAC AGCTTAAGTA AGGATGTAAG CCGCCTAGAA
2401     ACTGTGTTTG TACCTCCTGA GAAACATGAA AAAGAGATAA TAGCTCTGAA ATCCAATATT
2461     GTTGAACTTA AGAAACAGCT GTCTGAACTT AAGAAAAAAT GTGGTGAAGA CCAGGAGAAA
2521     ATACACGCTC TCACATCTGA AAACACTAAC TTGAAGAAGA TGATGAGTAA TCAGTATGTG
2581     CCAGTTAAAA CCCATGAAGA GGTTAAAATG ACACTGAATG ACACGTTAGC CAAAACTAAC
2641     AGAGAATTAT TAGATGTGAA GAAAAAATTT GAAGATATAA ATCAGGAATT TGTAAAAATA
2701     AAAGATAAGA ATGAAATATT AAAAAGAAAC CTGGAAAACA CTCAGAACCA AATAAAAGCT
2761     GAGTACATCA GCCTGGCAGA GCACGAGGCA AAGATGAGCT CGCTAAGTCA GAGCATGAGA
2821     AAGGTGCAGG ATAGTAATGC TGAAATCTTG GCCAACTACA GAAAAGGCCA AGAAGAGATT
2881     GTGACACTGC ATGCCGAAAT TAAAGCCCAG AAGAAGGAGC TCGACACAAT ACAAGAATGC
2941     ATTAAGGTAA AATATGCCCC AATTGTCAGC TTTGAGGAGT GCGAGAGAAA ATTTAAAGCA
3001     ACAGAGAAAG AACTAAAAGA CCAGTTATCA GAGCAGACAC AAAAGTATAG TGTCAGTGAA
3061     GAAGAAGTCA AGAAAAACAA GCAAGAGAAT GACAAGTTAA AGAAGGAGAT TTTTACCCTT
3121     CAGAAAGATT TGAGAGATAA GACAGTTCTC ATTGAGAAGT CTCATGAAAT GGAAAGAGCA
3181     TTAAGCAGAA AAACAGACGA GCTAAACAAA CAGTTAAAAG ACTTGTCACA GAAATACACG
3241     GAAGTAAAGA ATGTGAAAGA GAAGCTAGTA GAAGAAAATG CCAAACAGAC TTCTGAGATA
3301     CTTGCAGTGC AAAATCTTTT GCAAAAACAA CATGTTCCAT TGGAACAGGT TGAGGCTCTG
3361     AAAAAATCTC TTAATGGCAC AATTGAAAAT CTAAAGGAAG AACTGAAGAG TATGCAAAGG
3421     TGTTACGAGA AAGAGCAGCA GACAGTGACC AAACTGCATC AATTGTTGGA GAATCAAAAG
3481     AACTCTTCTG TACCCCTGGC AGAGCATTTG CAGATTAAAG AAGCATTTGA GAAAGAAGTT
3541     GGAATCATAA AAGCCAGCTT GAGAGAAAAG GAAGAAGAAA GCCAAAACAA AATGGAAGAA
3601     GTCTCCAAAC TTCAGTCGGA GGTTCAGAAT ACTAAACAAG CATTAAAAAA ATTAGAGACT
3661     AGAGAGGTAG TTGACTTGTC TAAATATAAA GCAACAAAAA GTGATTTGGA GACACAGATT
3721     TCTAGCTTAA ATGAAAAATT GGCCAATCTG AATAGAAAGT ATGAGGAAGT ATGTGAGGAA
3781     GTTTTGCATG CCAAAAAGAA GGAAATATCT GCAAAAGATG AGAAGGAATT ACTGCATTTC
3841     AGCATTGAGC AAGAAATTAA GGATCAGAAG GAACGATGTG ATAAGTCCTT AACAACAATC
3901     ACAGAGTTAC AAAGAAGAAT ACAAGAATCT GCTAAACAAA TAGAAGCAAA AGATAATAAG
3961     ATAACTGAAC TGCTTAATGA TGTGGAAAGA TTAAAACAGG CACTCAATGG CCTTTCCCAA
4021     CTCACCTACA CAAGTGGGAA CCCCACCAAG AGGCAGAGCC AGCTGATTGA CACTCTGCAG
4081     CACCAAGTGA AATCTCTGGA GCAACAGCTG GCCGATGCTG ACAGACAGCA CCAAGAAGTA
4141     ATTGCAATTT ATCGGACACA CCTTCTTAGT GCTGCACAGG GTCACATGGA TGAAGATGTT
4201     CAGGAGGCTC TGCTCCAGAT CATACAAATG CGGCAGGGGC TTGTGTGCTA G

Quality Control:cDNA sequencing results is available upon request.