Mouse TH Gene cDNA Clone / ORF Clone

TH cDNA Clone Datasheet

TH cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009377.1
• cDNA Size: 1497
• Gene Synonym: Th
• Source: Mouse

TH cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tyrosine hydroxylase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 833 A/G resulting in the amino acid his substitution by arg.

TH cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TH cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCACCC CCAGCGCCTC CTCGCCACAG CCCAAGGGCT TCAGAAGAGC CGTCTCAGAG
61       CAGGATACCA AGCAGGCCGA GGCTGTCACG TCCCCAAGGT TCATTGGACG GCGGCAGAGT
121      CTCATCGAGG ATGCCCGCAA GGAGCGGGAG GCAGCAGCAG CTGCAGCAGC GGCTGCGGTA
181      GCCTCCGCGG AACCTGGGAA CCCATTGGAG GCTGTGGTAT TCGAGGAGAG GGATGGAAAT
241      GCTGTTCTCA ACCTGCTCTT CTCCTTGAGG GGTACAAAAC CCTCCTCACT GTCTCGGGCT
301      TTGAAAGTGT TTGAGACATT TGAAGCCAAA ATCCACCACT TAGAGACCCG GCCTGCCCAG
361      AGGCCACTGG CAGGAAGCCC CCACCTGGAG TACTTTGTGC GCTTCGAGGT GCCCAGTGGC
421      GACCTGGCTG CCCTCCTCAG TTCTGTGCGT CGGGTGTCTG ACGATGTGCG CAGTGCCAGA
481      GAGGACAAGG TTCCCTGGTT CCCAAGGAAA GTGTCAGAGT TGGATAAGTG TCACCACCTG
541      GTCACCAAGT TTGACCCTGA CCTGGACCTG GACCATCCGG GCTTCTCTGA CCAGGCGTAT
601      CGCCAGCGCC GGAAGCTGAT TGCAGAGATT GCCTTCCAAT ACAAGCAGGG TGAGCCAATT
661      CCCCACGTGG AATACACAAA GGAGGAAATT GCTACCTGGA AGGAGGTATA CGCCACGCTG
721      AAGGGCCTCT ATGCTACCCA TGCCTGCCGG GAACACCTGG AGGCTTTCCA GCTTCTGGAA
781      CGGTACTGTG GCTACCGAGA GGACAGCATT CCACAGCTGG AGGATGTGTC TCGCTTCTTG
841      AAGGAACGGA CTGGCTTCCA GCTGCGACCC GTGGCCGGTC TACTGTCTGC CCGTGATTTT
901      CTGGCCAGTC TGGCCTTCCG TGTGTTTCAG TGCACACAGT ACATCCGTCA TGCCTCCTCA
961      CCTATGCACT CACCCGAGCC AGACTGCTGC CACGAGCTGC TGGGACACGT ACCCATGTTG
1021     GCTGACCGCA CATTTGCCCA GTTCTCCCAG GACATTGGAC TTGCATCTCT GGGGGCTTCA
1081     GATGAAGAAA TTGAAAAACT CTCCACGGTG TACTGGTTCA CTGTGGAGTT TGGGCTGTGT
1141     AAACAGAATG GGGAGCTGAA GGCTTACGGT GCAGGGCTGC TGTCTTCCTA TGGAGAGCTC
1201     CTGCACTCCC TGTCAGAGGA GCCCGAGGTC CGGGCCTTTG ACCCAGACAC AGCAGCCGTG
1261     CAGCCCTACC AAGATCAAAC CTACCAGCCG GTGTACTTCG TGTCAGAGAG CTTCAGTGAT
1321     GCCAAGGACA AGCTCAGGAA CTATGCCTCT CGTATCCAGC GCCCATTCTC TGTGAAGTTT
1381     GACCCGTACA CCCTGGCCAT TGATGTACTG GACAGTCCTC ACACCATCCG GCGCTCCTTA
1441     GAGGGGGTCC AGGATGAGCT GCACACCCTG ACCCAAGCAC TGAGTGCCAT TAGCTAA

 

Quality Control:cDNA sequencing results is available upon request.