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Tryptophan Hydroxylase 1 / TPH1 Antibody, Rabbit PAb, Antigen Affinity Purified

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TPH1Antibody Product Information
Antigen:Recombinant Human Tryptophan Hydroxylase 1 / TPH1 protein (Catalog#11931-H07E)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Tryptophan Hydroxylase 1 / TPH1 (rh Tryptophan Hydroxylase 1 / TPH1; Catalog#11931-H07E; P17752-1; Ile 2-Ile 444). Tryptophan Hydroxylase 1 / TPH1 specific IgG was purified by Human Tryptophan Hydroxylase 1 / TPH1 affinity chromatography.
TPH1Antibody Usage Guide
Specificity:Human Tryptophan Hydroxylase 1 / TPH1
Application:ELISA

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TPH1. The detection limit for Human TPH1 is approximately 0.00975 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme which belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells.The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.

References
  • Antonia S. New, et al.,1998, American Journal of Medical Genetics Part B. 81 (1): 13-17.
  • Y. C Wang, et al.,2001, Neuropsychobiology. 43 (1): 1 - 4.
  • Diego J. et al., 2003, Science. 299 (5603): 76.
  • Allen NC, et al.,2008, Nat Genet. 40 (7): 827-34.
  • Galfalvy,H. et al., 2009, J Affect Disord. 115 (3):331-8.
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