Human PRSS1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10816-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_002769.4

cDNA Size:   744

Synonym:  TRP1; TRY1; TRY4; TRYP1; MGC120175; MGC149362; PRSS1

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens protease, serine, 1 (trypsin 1) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations 30 G/T, 486 T/C and 738 T/C not causing the amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAATCCAC TCCTGATCCT TACCTTTGTT GCAGCTGCTC TTGCTGCCCC CTTTGATGAT
61       GATGACAAGA TCGTTGGGGG CTACAACTGT GAGGAGAATT CTGTCCCCTA CCAGGTGTCC
121      CTGAATTCTG GCTACCACTT CTGTGGTGGC TCCCTCATCA ACGAACAGTG GGTGGTATCA
181      GCAGGCCACT GCTACAAGTC CCGCATCCAG GTGAGACTGG GAGAGCACAA CATCGAAGTC
241      CTGGAGGGGA ATGAGCAGTT CATCAATGCA GCCAAGATCA TCCGCCACCC CCAATACGAC
301      AGGAAGACTC TGAACAATGA CATCATGTTA ATCAAGCTCT CCTCACGTGC AGTAATCAAC
361      GCCCGCGTGT CCACCATCTC TCTGCCCACC GCCCCTCCAG CCACTGGCAC GAAGTGCCTC
421      ATCTCTGGCT GGGGCAACAC TGCGAGCTCT GGCGCCGACT ACCCAGACGA GCTGCAGTGC
481      CTGGACGCTC CTGTGCTGAG CCAGGCTAAG TGTGAAGCCT CCTACCCTGG AAAGATTACC
541      AGCAACATGT TCTGTGTGGG CTTCCTTGAG GGAGGCAAGG ATTCATGTCA GGGTGATTCT
601      GGTGGCCCTG TGGTCTGCAA TGGACAGCTC CAAGGAGTTG TCTCCTGGGG TGATGGCTGT
661      GCCCAGAAGA ACAAGCCTGG AGTCTACACC AAGGTCTACA ACTATGTGAA ATGGATTAAG
721      AACACCATAG CTGCCAACAG CTAA

Quality Control: cDNA sequencing results is available upon request.