Human TrkB transcript variant b Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10047-M	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001007097.1

cDNA Size: 1434

Synonym:   NTRK2, TRKB, GP145-TrkB

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens neurotrophic tyrosine kinase receptor, type 2, transcript variant b DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGTCGTCCT GGATAAGGTG GCATGGACCC GCCATGGCGC GGCTCTGGGG CTTCTGCTGG
61       CTGGTTGTGG GCTTCTGGAG GGCCGCTTTC GCCTGTCCCA CGTCCTGCAA ATGCAGTGCC
121      TCTCGGATCT GGTGCAGCGA CCCTTCTCCT GGCATCGTGG CATTTCCGAG ATTGGAGCCT
181      AACAGTGTAG ATCCTGAGAA CATCACCGAA ATTTTCATCG CAAACCAGAA AAGGTTAGAA
241      ATCATCAACG AAGATGATGT TGAAGCTTAT GTGGGACTGA GAAATCTGAC AATTGTGGAT
301      TCTGGATTAA AATTTGTGGC TCATAAAGCA TTTCTGAAAA ACAGCAACCT GCAGCACATC
361      AATTTTACCC GAAACAAACT GACGAGTTTG TCTAGGAAAC ATTTCCGTCA CCTTGACTTG
421      TCTGAACTGA TCCTGGTGGG CAATCCATTT ACATGCTCCT GTGACATTAT GTGGATCAAG
481      ACTCTCCAAG AGGCTAAATC CAGTCCAGAC ACTCAGGATT TGTACTGCCT GAATGAAAGC
541      AGCAAGAATA TTCCCCTGGC AAACCTGCAG ATACCCAATT GTGGTTTGCC ATCTGCAAAT
601      CTGGCCGCAC CTAACCTCAC TGTGGAGGAA GGAAAGTCTA TCACATTATC CTGTAGTGTG
661      GCAGGTGATC CGGTTCCTAA TATGTATTGG GATGTTGGTA ACCTGGTTTC CAAACATATG
721      AATGAAACAA GCCACACACA GGGCTCCTTA AGGATAACTA ACATTTCATC CGATGACAGT
781      GGGAAGCAGA TCTCTTGTGT GGCGGAAAAT CTTGTAGGAG AAGATCAAGA TTCTGTCAAC
841      CTCACTGTGC ATTTTGCACC AACTATCACA TTTCTCGAAT CTCCAACCTC AGACCACCAC
901      TGGTGCATTC CATTCACTGT GAAAGGCAAC CCCAAACCAG CGCTTCAGTG GTTCTATAAC
961      GGGGCAATAT TGAATGAGTC CAAATACATC TGTACTAAAA TACATGTTAC CAATCACACG
1021     GAGTACCACG GCTGCCTCCA GCTGGATAAT CCCACTCACA TGAACAATGG GGACTACACT
1081     CTAATAGCCA AGAATGAGTA TGGGAAGGAT GAGAAACAGA TTTCTGCTCA CTTCATGGGC
1141     TGGCCTGGAA TTGACGATGG TGCAAACCCA AATTATCCTG ATGTAATTTA TGAAGATTAT
1201     GGAACTGCAG CGAATGACAT CGGGGACACC ACGAACAGAA GTAATGAAAT CCCTTCCACA
1261     GACGTCACTG ATAAAACCGG TCGGGAACAT CTCTCGGTCT ATGCTGTGGT GGTGATTGCG
1321     TCTGTGGTGG GATTTTGCCT TTTGGTAATG CTGTTTCTGC TTAAGTTGGC AAGACACTCC
1381     AAGTTTGGCA TGAAAGGTTT TGTTTTGTTT CATAAGATCC CACTGGATGG GTAG

Quality Control: cDNA sequencing results is available upon request.

TrkB cDNA Clone Related Products &Topics

Neuroscience>>Neurotrophic Factor & Receptor>>Neurotrophin & Receptor>>TrkB/NTRK2

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>TrkB/NTRK2

Cancer>>Growth Factor & Receptor>> Neurotrophin & Receptor>>TrkB/NTRK2

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>TrkB/NTRK2

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
TrkB/NTRK2	Human	TrkB/NTRK2/Fc Protein, Recombinant	10047-H03H
TrkB/NTRK2	Human	TrkB/NTRK2 Protein, Recombinant	10047-H08H
TrkB/NTRK2	Mouse	TrkB/NTRK2 Protein, Recombinant	50132-M08H
TrkB/NTRK2	Rat	NTRK2 / TrkB Protein, Recombinant	80243-R02H
TrkB/NTRK2	Rat	NTRK2 / TrkB Protein, Recombinant	80243-R08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human TrkB/NTRK2	WB	Mouse Monoclonal Antibody	10047-MM05
Human TrkB/NTRK2	ELISA	NTRK2 / Trk-B / TRKB Antibody	10047-MM12
Human TrkB/NTRK2	WB, ELISA, IHC	TrkB/NTRK2 Antibody, Rabbit MAb	10047-R107
Human TrkB/NTRK2	WB, ELISA	Rabbit Polyclonal Antibody	10047-RP01
Human TrkB/NTRK2	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10047-RP02

ELISA Kits:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human TrkB/NTRK2	ELISA	TrkB/NTRK2 ELISA Kit	SEK10047

cDNA Clones:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
TrkB/NTRK2	Mouse	Mus musculus TrkB/NTRK2 cDNA Clone	MG50132-M
TrkB/NTRK2	Canine	Canine NTRK2 Gene cDNA Clone / ORF Clone	DG70035-G
TrkB/NTRK2	Rat	Rat NTRK2 cDNA Clone / ORF Clone	RG80243-G

TrkB Gene Description

TrkB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor exclusively expressed in neurons of the central nervous system. BDNF(brain-derived neurotrophic factor) exerts biological actions through its physiological receptor TrkB which leads to the initiation of intracellular signaling cascades and different biological responses in neurons. TRKB has two distinct binding regions for BDNF within the extracellular domain with high affinity. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. Upon neurotrophin binding, TrkB phosphorylates itself and the downstream molecular members of the related signal pathways. TrkB has been shown to play an important role in tumorigenesis and metastasis, and mutations in this gene are associated with obesity and mood disorders. Accordingly, BDNF/TrkB signaling is thought to be a potential therapeutic target for schizophrenia.

References

Klein, R. et al., 1990, Cell. 64: 647-656.
Haniu, M. et al., 1997, J. Biol. Chem. 272 : 25296-25303.
Haapasalo, A. et al., 2002, J. Biol. Chem. 277: 43160-43167.
Pillai, A., 2008, Neurosignals. 16: 183-193.
Thomas, R. et al., 2007, Cancer. Res. 67: 6221-6229.

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