Anti-TrkB Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
No cross-reactivity in ELISA with
Human Trk-A / NTRK1 / TRK
Human Trk-C / NTRK3 / TRKC
Mouse Trk-C / NTRK3 / TRKC
Recombinant Human Trk-B protein (Catalog#10047-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NTRK2 / Trk-B extracellular domain (rh Trk-B; Catalog#10047-H08H; NP_001007098.1; Met 1-His 430). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Monoclonal Mouse IgG1 Clone #7H6E7B3
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TrkB Antibody (Mouse Monoclonal antibody) Validated Applications
||This antibody will detect Human TrkB / NTRK2 in ELISA pair set (Catalog: # SEK10047). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10047-MM01).
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-TrkB Antibody (Mouse Monoclonal antibody) Images
Flow cytometric analysis of Human TRKB expression on DU145 cells. Cells were stained with purified anti-Human TRKB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-TrkB Antibody Alternative Names
Anti-GP145-TrkB Antibody;Anti-GP145-TrkB/GP95-TrkB Antibody;Anti-Tkrb Antibody;Anti-trk-B Antibody;Anti-TRKB Antibody
TrkB Background Information
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
neurotrophic tyrosine kinase, receptor, type 2
Klein R, et al. (1990) The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 61 (4): 647-56. Rose CR, et al. (2003) Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells. Nature. 426 (6962): 74-8. Yamada K, et al. (2004) Brain-derived neurotrophic factor/TrkB signaling in memory processes. J Pharmacol Sci. 91 (4): 267-70.