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TrkB / NTRK2 Antibody, Mouse MAb

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Human TrkB/NTRK2 Antibody Product Information
Immunogen:Recombinant Human Trk-B protein (Catalog#10047-H08H)
Clone ID:7H6E7B3
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NTRK2 / Trk-B extracellular domain (rh Trk-B; Catalog#10047-H08H; NP_001007098.1; Met 1-His 430). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
Human TrkB/NTRK2 Antibody Usage Guide
Specificity:Human NTRK2 / Trk-B / TrkB
No cross-reactivity in WB and ELISA with
Human Trk-A / NTRK1 / TRK
Human Trk-C / NTRK3 / TRKC
Mouse Trk-C / NTRK3 / TRKC
Human cell lysate (293 cell line)
Application:ELISA, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Trk-B. The detection limit for Human Trk-B is 0.0195 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TrkB/NTRK2 Antibody FC Application Image
TrkB / NTRK2 Antibody, Mouse MAb
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human TRKB expression on DU145 cells. Cells were stained with purified anti-Human TRKB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other TrkB/NTRK2 Antibody Products
Reactivity: Human  
Application: FCM  
  • Slide 1
10047-MM12-P-50
10047-MM12-P-100
50 Tests 
100 Tests 
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Reactivity: Human  
Application: ELISA  
    10047-RP01-400
    10047-RP01-100
    10047-RP01-200
     
    100 µg 
    200 µg 
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    Reactivity: Human  
    Application: ELISA  
      10047-RP02-200
      10047-RP02-50
      10047-RP02-100
      200 µg 
      50 µg 
      100 µg 
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      Reactivity: Human  
      Application: ELISA  
        10047-R107-50
        10047-R107-100
        50 µg 
        100 µg 
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        TrkB/NTRK2 Background

        TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.

        Human TrkB/NTRK2 References
      • Klein R, et al. (1990) The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 61 (4): 647-56.
      • Rose CR, et al. (2003) Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells. Nature. 426 (6962): 74-8.
      • Yamada K, et al. (2004) Brain-derived neurotrophic factor/TrkB signaling in memory processes. J Pharmacol Sci. 91 (4): 267-70.
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        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"