Transferrin / TF Antibody

Transferrin / TF Antibody Datasheet

Transferrin / TF Antibody Product Information

• Immunogen: Recombinant Human Transferrin / TF protein (Catalog#11019-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 101
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin / TF (rh Transferrin / TF; Catalog#11019-H08H; NP_001054.1; Met 1-Pro 698).

Transferrin / TF Antibody Usage Guide

• Specificity: Human Transferrin / TF
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human Transferrin / TF in WB. Using a DAB detection system, the detection limit for Human Transferrin / TF is approximately 2 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Transferrin / TF. The detection limit for Human Transferrin / TF is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Transferrin / TF Antibody Background

Transferrin, also known as Serotransferrin, Beta-1 metal-binding globulin, TF, is a secreted protein which belongs to thetransferrin family. It is expressed by the liver and secreted in plasma and contains two transferrin-like domains. Transferrins are iron binding transport proteins which can bind two Fe3+ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin protein loaded with iron encounters atransferrin receptoron the surface of acell, it binds to it and, as a consequence, is transported into the cell in avesicle. Transferrin is aglycoproteinthat binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Defects in transferrin are the cause of a transferrinemia (ATRAF) which is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Transferrin is also associated with the innate immune system.

References

1. Cheng Y, et al., 2004, Cell116 (4): 565-76.
2. St. Edward's University. 2005-07-18. Retrieved 2009-04-24.
3. Hafenstein S, et al., 2007,PNAS. 104 (16): 6585-9.