Transferrin Receptor / TFRC / CD71 Antibody

Transferrin Receptor / TFRC / CD71 Antibody Datasheet

Transferrin Receptor / TFRC / CD71 Antibody Product Information

• Immunogen: Recombinant Human Transferrin Receptor / TFRC / CD71 protein (Catalog#11020-H07H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; Catalog#11020-H07H; NP_003225.2; Cys89-Phe 760). Total IgG was purified by Protein A affinity chromatography.

Transferrin Receptor / TFRC / CD71 Antibody Usage Guide

• Specificity: Human Transferrin Receptor / TFRC / CD71
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TFRC in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TFRC. The detection limit for Human TFRC is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Transferrin Receptor / TFRC / CD71 Antibody Background

Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p90, CD71 and TFRC, is a single-pass type II membrane protein which belongs to thepeptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.

References

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4. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.