Transferrin / TF Protein (His Tag)

Serotransferrin / Transferrin / TF Protein Datasheet

Serotransferrin / Transferrin / TF Protein Product Information

• Synonym: TF , DKFZp781D0156 , PRO1557 , PRO2086
• Protein Construction: A DNA sequence encoding the human transferrin ( NP_001054.1 ) ( Met 1 - Pro 698 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

Serotransferrin / Transferrin / TF Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized human CD71 at 10 μg/ml ( 100 μl/well ) can bind human Transferrin
  The EC₅₀ of human Transferrin is 5.6 ng/mL
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 20
• Molecular Mass: The secreted recombinant human transferrin comprises 690 amino acids with a predicted molecular mass of 76.6 kDa. It migrates as an approximately 74 kDa band in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Transferrin protein

Serotransferrin / Transferrin / TF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Serotransferrin / Transferrin / TF Protein Description

Transferrin, also known as Serotransferrin, Beta-1 metal-binding globulin, TF, is a secreted protein which belongs to the transferrin family. It is expressed by the liver and secreted in plasma and contains two transferrin-like domains. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin protein loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and, as a consequence, is transported into the cell in a vesicle. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Defects in transferrin are the cause of a transferrinemia (ATRAF) which is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Transferrin is also associated with the innate immune system.

References

1. Cheng Y, et al., 2004, Cell 116 (4): 565-76. 
2. St. Edward's University. 2005-07-18. Retrieved 2009-04-24.
3. Hafenstein S, et al., 2007, PNAS. 104 (16): 6585-9.