Transferrin / TF / Serotransferrin Antibody (Antigen Affinity Purified)

Transferrin / TF Antibody ( Antigen Affinity Purified )

Transferrin / Serotransferrin / TF Antibody Product Information

• Immunogen: Recombinant human transferrin protein ( Catalog#11019-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human Transferrin / TF ( rh Transferrin ; Catalog#11019-H08H ; NP_001054.1 ; Met 1- Pro 698 ). Transferrin specific IgG was purified by human Transferrin affinity chromatography.

Transferrin / Serotransferrin / TF Antibody Usage Guide

• Specificity: Human Transferrin / Serotransferrin / TF
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human transferrin in WB. Using a DAB detection system, the detection limit for human transferrin is approximately 0.5 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect human transferrin. The detection limit for human transferrin is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Transferrin / Serotransferrin / TF Antibody Background

Transferrin, also known as Serotransferrin, Beta-1 metal-binding globulin, TF, is a secreted protein which belongs to the transferrin family. It is expressed by the liver and secreted in plasma and contains two transferrin-like domains. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin protein loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and, as a consequence, is transported into the cell in a vesicle. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Defects in transferrin are the cause of a transferrinemia (ATRAF) which is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Transferrin is also associated with the innate immune system.

References

1. Cheng Y, et al., 2004, Cell 116 (4): 565-76. 
2. St. Edward's University. 2005-07-18. Retrieved 2009-04-24.
3. Hafenstein S, et al., 2007, PNAS. 104 (16): 6585-9.