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Sinofection® Transfection Reagent

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Introduction to Sinofection

Sinofection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Sinofection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.

Sinofection Features
  • For eukaryotic cells like HEK 293;
  • For both adherent and suspension cells;
  • For operations with or without serum;
  • More than 200,000 L application.

High protein expression and low cytotoxicity(EGFP protein expression transfected Sinofection )

293E 293FT
  COS7 HepG2
  SW480 CHO-DG44
Sinofection Transfection Protocol
The transfection procedures of adherent cells are shown in Fig. 1.
we take the 96-well plates as example:
1. Preparation: Cells are seeded in plates at 1.5-4.5×104cells/well (table 1)and cultured with 5%CO2 at 37℃ for 18-24h before transfection.
2. Preparation of transfection mixture:
(1). Dilute DNA into serum-free medium(25μL in total) and homogenize gently.
(2). Dilute Sinofection into serum-free medium(25μL in total) and homogenize gently and incubate at room temperature for 5 min.
(3). Mix the DNA and Sinofection at room temperature for 15~20min.
3. Remove the culture medium and add 50μL mixture per well.
4. After 4-6hours(for SF9 cell is 2h), remove the transfection mixture and add medium with serum.
5. Gene expression is tested after incubation with 5% CO2 at 37℃ for 48-72h(incubate sf9 cell line at 27℃ for 48-72h).

Fig. 1 Adherent cells' Transfection

(1). The experiment conditions for different cell lines are shown in Table 1. The corresponding transfection protocols can be got by entering links in Table 1.
(2). Number of cells per well, dosage of Sinofection, DNA and serum-free medium for dilution are proportional to  basal area per well for plates of different sizes. The basical areas for plates with different sizes are shown in Table 2. The dosage and ratio of DNA and Sinofection should be optimized to achieve the best transfection results.

Table1. Usage of Sinofection in different cell lines(96-well plate)

Cell type Culture medium Cells per well DNA Sinofection Medium change after 4-6h
293H DMEM 3×104 0.2µg 0.5µL DMEM+10%FBS
293FT DMEM 3×104 0.2µg 0.5µL DMEM+10%FBS
293E DMEM 3×104 0.2µg 0.5µL DMEM+10%FBS
293F DMEM 3×104 0.2µg 0.5µL DMEM+10%FBS
COS7 DMEM 1.5×104 0.4µg 0.5µL DMEM+10%FBS
hela DMEM 2×104 0.3µg 0.5µL 1640+15%FBS
Caco2 MEM 3.5×104 0.3µg 0.75µL MEM+10%FBS
BHK21 MEM 2×104 0.2µg 0.5µL MEM+10%FBS
CHO-DG44 DMEM+HT+pro 2×104 0.5µg 0.5µL DMEM+HT+pro +10%FBS
RAW264.7 DMEM 3×104 0.2µg 0.5µL DMEM +10%FBS
MCF7 MEM/NEAA+0.01mg/mL insulin + sodium pyruvat 2×104 0.1µg 0.25µL MEM/NEAA+0.01mg/mL insulin + sodium pyruvat+10%FBS
SW480 IMDM 3×104 0.4µg 0.5µL IMDM +10%FBS
MDCK DMEM 4×104 0.6µg 1µL DMEM+10%FBS
CHO-K1 IMDM+Pro 3×104 0.2µg 0.5µL IMDM+Pro +10%FBS
HepG2 DMEM 3×104 0.5µg 0.75µL DMEM+10%FBS
A549 DMEM 2×104 0.3µg 0.5µL DMEM+10%FBS
NIH/3T3 DMEM 1.5×104 0.1µg 0.75µL DMEM+10%FBS
vero DMEM 3×104 0.3µg 0.75µL DMEM+10%FBS
sf9 SIM SF 5×104 0.4µg 0.75µL SIM SF+10%FBS

Table 2. Scaling up or down transfections with Sinofection(according to the growth area).
Note: the actual conditions should be optimized by experiments.

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