Tie2 / CD202b / TEK Protein (GST Tag)

Tyrosine kinase, endothelial Protein Datasheet

Tie2 / CD202b / TEK Protein Product Information

• Synonym: TEK, TIE2, VMCM, VMCM1
• Protein Construction: A DNA sequence encoding the human TEK(NP_000450)(Gln771-Ala1124)was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

Tie2 / CD202b / TEK Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized human TEK (aa 771-1124) (Cat: 10700-H20B1) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc (Cat: 10691-H02H) with a linear range of 0.31-20 μg/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human TEK /GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0,10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Tie2 / CD202b / TEK protein

Tie2 / CD202b / TEK Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Tie2 / CD202b / TEK Protein Description

Tie2/Tek, together with Tie1/Tie, are members of the receptor tyrosine kinase (RTK) subfamily, and possess a unique extracellular domain containing two immunoglobulin-like loop domains flanking three epidermal growth factor (EGF) repeats followed by three fibronectin-type I II repeats, and a split tyrosine kinase domain in the cytoplasmic region. Tie2 is expressed predominantly on endothelial and hematopoietic progenitor cells and is essential for blood vessel formation and maintenance. The identified ligand angiopoietin-1 (Ang-1) binds Tie2 receptor with high-affinity and induces the signaling pathway of both cell migration and cell survival via the related mediator such as GRB2 and SH-PTP2. Accordingly, the Tie2 may trigger crucial endothelial responses during embryonic vascular development and during pathologic vascular growth, as well as the inflammatory responses with the key regulator ABIN-2. Recent study has revealed that Ang1-Tie2 signaling exerts different function at cell-cell contacts and cell-substratum contacts dependent upon the spatial localization of Tie2. Deficiency in this gene is associated with inherited venous malformations.

References

1. Partanen J. et al., 1992, Mol Cell Biol. 12: 1698-707.
2. Sato TN. et al., 1993, Proc. Natl. Acad. Sci. 90: 9355-8.
3. Jones N. et al., 1999, J. Biol. Chem. 274: 30896-905.
4. Fukuhara S. et al., 2008, Nat. Cell. Biol. 10: 513-26.
5. Marron MB. et al., 2007, J Biol Chem. 282: 30509-17.
6. Huqhes DP. et al., 2003, Circ Res. 92: 630-6.