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Thioredoxin / TXN Antibody Datasheet
|Order or Inquire for Thioredoxin / TXN Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.00245 ng/well in ELISA|
Thioredoxin / TXN Antibody Product Information
Recombinant Human Thioredoxin / TXN protein (Catalog#10384-HNAE)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; Catalog#10384-HNAE; P10599; Met 1-Val 105). Total IgG was purified by Protein A affinity chromatography.
Thioredoxin / TXN Antibody Usage Guide
Human Thioredoxin / TXN
|Western blot :||This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TXN in WB.|
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TXN. The detection limit for Human TXN is approximately 0.00245 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Thioredoxin / TXN Antibody Related Products & Topics
Thioredoxin / TXN Antibody Background
Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to thethioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
- Holmgren A, et al.,1989, J Biol Chem264 (24): 13963-6.
- Nordberg J, et al., 2001,Free Radic Biol Med31 (11): 1287-312.
- Mustacich D, et al., 2000, Biochem J346 (Pt 1): 1-8.
- Mitchell D.A.,et al., 2005, Nat. Chem. Biol. 1:154-158.
Thioredoxin (TXN) related areas, pathways, and other information