Anti-Thioredoxin Antibody

Cat: 10384-R116
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Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Thioredoxin Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Thioredoxin
Recombinant Human Thioredoxin / TXN protein (Catalog#10384-HNAE)
This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; Catalog#10384-HNAE; P10599; Met1-Val105).
Monoclonal Rabbit IgG Clone #116
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:2000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) Images
Human Thioredoxin/TXN/SASP Western blot (WB) 13208

Anti-TXN rabbit monoclonal antibody at 1:2000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: THP-1 Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:14 kDa

Human Thioredoxin/TXN/SASP Immunoprecipitation(IP) 17670

TXN was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-TXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TXN rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 13 kDa

Observed band size: 13 kDa

Anti-Thioredoxin Antibody Alternative Names
Anti-DKFZp686B1993 Antibody;Anti-MGC61975 Antibody;Anti-RP11-427L11.1 Antibody;Anti-TRDX Antibody;Anti-TRX Antibody;Anti-TRX1 Antibody;Anti-TXN Antibody
Thioredoxin Background Information

Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Full Name
  • Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6.
  • Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312.
  • Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 
  • Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Thioredoxin Antibody Rabbit Polyclonal ELISA 50822-RP01
    Anti-Thioredoxin Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 50822-T50
    Anti-Thioredoxin Antibody Rabbit Monoclonal WB,ELISA 50822-R002
    Anti-Thioredoxin Antibody Rabbit Monoclonal ELISA 50822-R092
    Anti-Thioredoxin Antibody Rabbit Monoclonal ELISA 10384-R177
    Anti-Thioredoxin Antibody Rabbit Polyclonal ELISA 10384-RP01
    Anti-Thioredoxin Antibody Rabbit Monoclonal IHC-P 10384-R214
    Anti-Thioredoxin Antibody Rabbit Polyclonal WB,ELISA,IP 10384-RP02
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