Anti-Thioredoxin Antibody

Cat: 10384-R116
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Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Thioredoxin Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Thioredoxin
Immunogen
Recombinant Human Thioredoxin / TXN protein (Catalog#10384-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; Catalog#10384-HNAE; P10599; Met1-Val105).
Source
Monoclonal Rabbit IgG Clone #116
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:2000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Thioredoxin Antibody (Rabbit Monoclonal antibody) Images
Human Thioredoxin/TXN/SASP Western blot (WB) 13208

Anti-TXN rabbit monoclonal antibody at 1:2000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: THP-1 Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:14 kDa

Human Thioredoxin/TXN/SASP Immunoprecipitation(IP) 17670

TXN was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-TXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TXN rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 13 kDa

Observed band size: 13 kDa

Anti-Thioredoxin Antibody Alternative Names
Anti-DKFZp686B1993 Antibody;Anti-MGC61975 Antibody;Anti-RP11-427L11.1 Antibody;Anti-TRDX Antibody;Anti-TRX Antibody;Anti-TRX1 Antibody;Anti-TXN Antibody
Thioredoxin Background Information

Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Full Name
thioredoxin
References
  • Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6.
  • Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312.
  • Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 
  • Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-Thioredoxin Antibody Rabbit Polyclonal ELISA 10384-RP01
    Anti-Thioredoxin Antibody Rabbit Monoclonal IHC-P 10384-R214
    Anti-Thioredoxin Antibody Rabbit Polyclonal WB,ELISA,IP 10384-RP02
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