Thioredoxin-2 / TXN2 Antibody

Thioredoxin-2 / TXN2 Antibody Datasheet

Thioredoxin-2 / TXN2 Antibody Product Information

• Immunogen: Recombinant Human Thioredoxin-2 / TXN2 protein (Catalog#12557-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Thioredoxin-2 / TXN2 (rh Thioredoxin-2 / TXN2; Catalog#12557-H07E; Q99757; Thr 60-Gly 166). Total IgG was purified by Protein A affinity chromatography.

Thioredoxin-2 / TXN2 Antibody Usage Guide

• Specificity: Human Thioredoxin-2 / TXN2
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TXN2 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TXN2. The detection limit for Human TXN2 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Thioredoxin-2 / TXN2 Antibody Background

Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains onethioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.

References

1. Chen Y., et al., 2002, J. Biol. Chem. 277: 33242-8.
2. Smeets A., et al., 2005, Protein Sci. 14: 2610-21.
3. Wen,S. et al., 2009, Am J Med Genet A149A (2): 155-60.
4. Choudhary C., et al., 2009, Science 325: 834-40.