Mouse TYMP Gene cDNA Clone / ORF Clone

TYMP cDNA Clone Datasheet

TYMP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_138302.1
• cDNA Size: 1416
• Gene Synonym: Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik; Tymp
• Source: Mouse

TYMP cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus thymidine phosphorylase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TYMP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TYMP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TYMP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGCTC CAGGGACACC ACCTCCCTCG GCCTCAGGGG GAGGAGGTGG GGAGCCCAGG
61       CAGCTGCCTG AGCTGATCCG CCTGAAGCGA GATGGAGGGC ATCTGAGAGA AGCAGATATC
121      AGAAACTTCG TGCACGCGGT GATAGATGGA AGAGCACAGG ACACACAAAT TGGGGCCATG
181      CTAATGGCCA TTCGGCTGCA GGGAATGAAC CTAGAAGAGA CATCTGTGCT GACTCGGGCC
241      CTTGCAGAGT CTGGGCAGCA ACTGGAGTGG CCCAAAGCCT GGCACCAGCA GCTTGTTGAC
301      AAACACTCCA CAGGAGGTGT GGGTGACAAG GTTAGCCTGG TTCTGGCACC TGCCCTGGCT
361      GCATGCGGCT GTAAGGTGCC AATGATCAGC GGCCGCAGTC TGGGACACAC AGGGGGCACA
421      CTGGATAAGC TGGAATCGAT TCCTGGGTTC GGTGTCACCC AGAGCCCAGA GCAGATGCTG
481      CACATCCTTG AGGAAGTCGG CTGCTGCATT GTCGGCCAGA GTGCAAAGCT GGTTCCTGCT
541      GATGGGATCC TGTATGCTGC ACGAGATGTG ACAGCCACTG TGGACAGTGT CCCACTCATT
601      ACAGCTTCAA TCCTCAGTAA GAAGGCCGTG GAGGGACTGT CCACTCTGGT GGTAGATGTT
661      AAGTTTGGGG GAGCTGCAGT CTTCCCAGAC CAGGAGAAGG CCCGAGAACT GGCAAAGATG
721      TTGGTTAGAG TGGGAGTGAG CCTGGGGCTG AAGGTCGCCG CAGCCCTGAC CGCCATGGAT
781      AACCCTCTGG GCCGCAGCGT GGGCCATACC CTGGAGGTGG AAGAAGCGTT GCTTTGCCTG
841      GATGGTGCGG GGCCGCCGGA TCTGCGGGAC CTGGTCATTA GGCTAGGAGG CGCCATTCTT
901      TGGATTAGCG GACAGGCGGA AACACAAGAC CAAGGCGCCG CCCGAGTGGC CGCGGCGTTG
961      GATGACGGCT CCGCGCGGCG CCGCTTCCAG CTGATGCTTT CGGCGCAGGG CGTGGACCCC
1021     GGCCTAGCTA AAGCATTGTG CTCCGGGAGC CCCACGCAAC GCCGGCAGCT GCTGCCTCAC
1081     GCCCGAGAGC AAGAGGAGCT GCTCGCACCG GCAGACGGCA TTGTTGAGTG CGTCCGAGCG
1141     CTGCCGCTGG CCCGTGTGCT GCACGACCTC GGAGCCGGAC GCAGCCGAGC GGGGCAGCCG
1201     ATCAGACCGG GAGTGGGTGC CGAGGTACTG GTCGACGTGG GCCAGTGTTT GTCCCGAGGG
1261     ACACCCTGGC TCCGCGTGCA CCTAGACGGC CCTGCGCTCA GCAGTCAGCA GCGACGCACT
1321     CTCCAGGGGG CGCTCGTGCT ATCAGATCGA GCACCCTTCA AGGTCCCTTC ACCCTTCGCT
1381     GAACTTGTCC TCCCACCGAC CATCGCACAG CCCTAA

 

Quality Control:cDNA sequencing results is available upon request.