TXNRD1 / GRIM-12 Antibody (Antigen Affinity Purified)

TXNRD1 / GRIM-12 Antibody ( Antigen Affinity Purified ) Datasheet

TXNRD1 / GRIM-12 Antibody Product Information

• Immunogen: Recombinant Human TXNRD1 / GRIM-12 protein (Catalog#13093-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human TXNRD1 / GRIM-12 (rh TXNRD1 / GRIM-12; Catalog#13093-H07E; Q16881-1; Tyr 161-Cys647). TXNRD1 / GRIM-12 specific IgG was purified by Human TXNRD1 / GRIM-12 affinity chromatography.

TXNRD1 / GRIM-12 Antibody Usage Guide

• Specificity: Human TXNRD1 / GRIM-12
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TXNRD1 in WB. Using a DAB detection system, the detection limit for Human TXNRD1 is approximately 4 ng/lane under non-reducing conditions and rreducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TXNRD1. The detection limit for Human TXNRD1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TXNRD1 / GRIM-12 Antibody Background

Thioredoxin reductase 1, also known as Gene associated with retinoic and interferon-induced mortality 12 protein, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein, KM-102-derived reductase-like factor, Thioredoxin reductase TR1, TXNRD1 and GRIM12, is a cytoplasm and nucleus which belongs to theclass-I pyridine nucleotide-disulfide oxidoreductase family. TXNRD1 / GRIM12 contains oneglutaredoxin domain. Isoform1 of TXNRD1 / GRIM12 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform4of TXNRD1 / GRIM12 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform5enhances the transcriptional activity of the beta receptor only. Isoform5of TXNRD1 / GRIM12 also mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 of TXNRD1 / GRIM12 is expressed predominantly in Leydig cells (at protein level). It is also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 of TXNRD1 / GRIM12 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

References

1. Gasdaska P.Y., et al., 1995,FEBS Lett. 373: 5-9.
2. Rundloef A.-K., et al., 2004, Free Radic. Biol. Med. 36: 641-656.
3. Rikova K., et al., 2007, Cell 131: 1190-1203.
4. Fritz-Wolf K., et al., 2007, J. Mol. Biol. 370: 116-127.
5. Choudhary C., et al., 2009, Science 325: 834-840.