Mouse TWF2 Gene cDNA Clone / ORF Clone

TWF2 cDNA Clone Datasheet

TWF2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011876.3
• cDNA Size: 1050
• Gene Synonym: Ptk9l; Ptk9r; AU014993; A6-related; Twf2
• Source: Mouse

TWF2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus twinfilin, actin-binding protein, homolog 2 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two: point mutation 126 A/G and 915 T/C not causing the amino acid variation.

TWF2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TWF2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TWF2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCACC AGACCGGCAT CCACGCCACT GAAGAGCTAA AGGAATTCTT TGCCAAGGCC
61       CGGGCTGGCT CCATCCGACT TATCAAAGTC ATCATTGAGG ACGAACAGCT CGTGCTGGGT
121      GCCTCGCAGG AGCCAGTGGG ACGCTGGGAC CAGGACTACG ACCGGGCTGT GCTGCCACTG
181      CTAGACGCCC AAGAGCCCTG CTACCTCCTC TTCCGACTTG ATTCGCAGAA TGCTCAGGGT
241      TTCGAGTGGC TTTTCCTGGC CTGGTCACCT GACAATTCGC CGGTGCGGCT GAAGATGCTA
301      TATGCAGCCA CACGCGCCAC CGTGAAGAAG GAGTTTGGAG GCGGCCACAT CAAGGATGAG
361      CTCTTCGGGA CAGTAAAGGA TGACCTCTCC TTGGCTGGGT ACCAGAAGCA CCTGTCATCG
421      TGTGCCGCAC CTGCCCCACT GACTTCTGCC GAGAGAGAAC TCCAGCAGAT CCGAATCAAT
481      GAGGTGAAGA CTGAGATCAG CGTGGAAAGT AAACACCAGA CGCTGCAGGG CCTGGCCTTC
541      CCTCTGCAGC CTGAGGCCCA GAGGGCACTT CAGCAACTCA AACAGAAGAC GGTCAACTAT
601      ATCCAGCTGA AGCTGGACCT GGAACGGGAG ACCATCGAGC TGGTACACAC AGAACCCACA
661      AATGTGGCCC AGCTGCCCTC ACGGATTCCT CGAGATGCTG CCCGCTACCA CTTCTTCCTA
721      TACAAGCATA CTCATGAGGG TGATGCCCTT GAATCTGTGG TGTTCATCTA CTCCATGCCC
781      GGGTACAAGT GCAGCATCAA GGAGCGGATG CTCTACTCCA GCTGCAAGAG CCGCCTCCTT
841      GACTCCGTAG AGCAAGACTT CCAGCTGGAG ATAGCTAAGA AGATTGAGAT CGGCGATGGA
901      GCAGAGCTGA CAGCCGAGTT CCTCTATGAC GAGGTACATC CCAAGCAACA CGCGTTCAAG
961      CAGGCGTTCG CCAAGCCCAA AGGCCCCGGG GGCAAGCGGG GCCACAAGCG CCTCATCCGG
1021     GGCCCCGGGG AGAACGGGGA AGACAGCTAG

 

Quality Control:cDNA sequencing results is available upon request.