Mouse TUBB3 Gene cDNA Clone / ORF Clone

TUBB3 cDNA Clone Datasheet

TUBB3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_023279.2
• cDNA Size: 1353
• Gene Synonym: M(beta)3, M(beta)6, 3200002H15Rik, Tubb3
• Source: Mouse

TUBB3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tubulin, beta 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

TUBB3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TUBB3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TUBB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGAGA TCGTGCACAT CCAGGCCGGC CAGTGCGGCA ACCAGATCGG GGCCAAGTTC
61       TGGGAAGTCA TCAGTGATGA GCATGGCATC GACCCCAGCG GCAACTACGT GGGCGACTCG
121      GACTTGCAGC TGGAGCGGAT CAGCGTCTAC TACAACGAGG CCTCTTCTCA CAAGTACGTG
181      CCTCGAGCCA TTCTGGTGGA CCTGGAACCC GGAACCATGG ACAGTGTCCG CTCAGGGGCC
241      TTTGGACATC TCTTCAGGCC TGACAATTTC ATCTTTGGTC AGAGTGGGGC CGGCAACAAC
301      TGGGCCAAGG GTCACTACAC GGAGGGGGCG GAGCTGGTGG ATTCGGTCCT GGATGTGGTG
361      CGGAAGGAGT GTGAAAACTG CGACTGCCTG CAGGGCTTCC AGCTGACCCA CTCGCTGGGG
421      GGCGGCACGG GCTCCGGCAT GGGCACGTTG CTCATCAGCA AGGTGCGTGA GGAGTATCCC
481      GACCGCATCA TGAACACCTT CAGCGTCGTG CCCTCACCCA AGGTGTCAGA CACGGTGGTG
541      GAGCCCTACA ACGCCACGCT GTCCATCCAC CAGCTGGTGG AGAACACGGA TGAGACCTAC
601      TGCATCGACA ACGAGGCGCT CTACGACATC TGCTTCCGCA CCCTCAAGCT GGCCACGCCC
661      ACCTACGGGG ACCTCAACCA CCTGGTATCG GCCACCATGA GCGGAGTCAC CACCTCCTTG
721      CGCTTCCCGG GCCAGCTCAA CGCTGACCTG CGCAAGCTGG CCGTCAACAT GGTGCCCTTC
781      CCGCGCCTGC ACTTCTTCAT GCCCGGCTTC GCCCCCCTCA CAGCCCGGGG CAGCCAGCAG
841      TACCGGGCCC TGACCGTGCC CGAGCTCACC CAGCAGATGT TCGATGCCAA GAACATGATG
901      GCCGCCTGCG ACCCGCGCCA CGGCCGCTAC CTGACGGTGG CCACCGTGTT CCGGGGCCGC
961      ATGTCCATGA AGGAGGTGGA CGAGCAGATG CTGGCCATCC AGAGCAAGAA CAGCAGCTAC
1021     TTCGTGGAGT GGATCCCCAA CAACGTGAAG GTGGCCGTGT GTGACATCCC GCCCCGCGGC
1081     CTCAAGATGT CCTCCACCTT CATCGGGAAC AGCACGGCCA TCCAGGAGCT GTTCAAGCGC
1141     ATCTCCGAGC AGTTCACGGC CATGTTCCGG CGCAAGGCCT TCCTGCACTG GTACACGGGC
1201     GAGGGCATGG ACGAGATGGA GTTCACCGAG GCCGAGAGCA ACATGAACGA CCTGGTGTCC
1261     GAGTACCAGC AGTACCAGGA CGCCACGGCC GAGGAAGAGG GCGAGATGTA CGAAGACGAC
1321     GAGGAGGAGT CGGAGGCCCA GGGCCCCAAG TGA

 

Quality Control:cDNA sequencing results is available upon request.