TRP1 / TYRP1 Protein (His Tag)

Tyrosinase-related protein 1 Protein Datasheet

TRP1 / TYRP1 Protein Product Information

• Synonym: RP11-3L8.1, CAS2, CATB, GP75, TRP, TYRP, b-PROTEIN
• Protein Construction: A DNA sequence encoding the human TYRP1 (P17643) extracellular domaqin (Met 1- Arg 471) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

TRP1 / TYRP1 Protein QC Testing

• Purity: >95% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 25
• Molecular Mass: The secreted recombinant human TYRP1 comprises 458 amino acids and has a predicted molecular mass of 52.2 kDa. The apparent molecular mass of rh TYRP1 is approximately 66 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TRP1 / TYRP1 protein

TRP1 / TYRP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TRP1 / TYRP1 Protein Description

Tyrosinase-related protein 1, also known as TYRP1 or TRP1, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Mutations in this enzyme are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type Ⅲ. Tyrosinase-related protein 1 (TYRP1) is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. This enzyme may regulate or influence the type of melanin synthesized. The expression of Tyrosinase-related protein 1 (TYRP1) is regulated by the microphthalmia-associated transcription factor (MITF). There is mounting evidence demonstrating that in addition to its role in eumelanin synthesis, TYRP1 is involved in maintaining stability of tyrosinase proliferation and melanocyte cell death.

References

1. Sarangarajan R. et al., 2001, Pigment Cell Res. 14(6): 437-44.
2. Box NF. et al., 1998, Genome. 9 (1): 50-3.