Mouse TROP2 / TACSTD2 Protein (His Tag)

Tumor-associated calcium signal transducer 2 Protein Datasheet

TROP2 / TACSTD2 Protein Product Information

• Synonym: C80403, EGP-1, GA733-1, Ly97, TROP2
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3) (Met 1-Gln 270) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

TROP2 / TACSTD2 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 25
• Molecular Mass: The secreted recombinant mouse TACSTD2 comprises 257 amino acids and has a calculated molecular mass of 29.4 kDa. As a result of glycosylation, the apparent molecular mass of rmTACSTD2 is approximately 45 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TROP2 / TACSTD2 protein

TROP2 / TACSTD2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TROP2 / TACSTD2 Protein Description

TROP2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed by human carcinomas. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. The cytoplasmic tail of TROP2 possesses potential serine and tyrosine phosphorylation sites and a conserved phosphatidyl-inositol binding consensus sequence. TROP2 transduces an intracellular calcium signal and acts as a cell surface receptor, meanwhile is involved in the regulation of cell- cell adhesion. Accordingly, as a widespread stimulator of human cancer growth and a unique marker and causal factor of metastatic cancer, TROP2 is a possible candidate for diagnosis and molecular target therapy. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.

References

1. Fornaro M. et al., 1995, Int J Cancer. 62: 610-8.
2. Ripani E. et al., 1998, Int J Cancer. 76: 671-6.
3. Linnenbach AJ. et al., 1993, Mol Cell Biol. 13: 1507-15.
4. Ohmachi T. et al., 2006, Clin Cancer Res. 12: 3057-63.
5. Tsujikawa M. et al., 1999, Nat Genet. 21: 420-3.