TROP2 / TACSTD2 Antibody ( FITC )

TROP2 / TACSTD2 Antibody Datasheet

TROP2 / TACSTD2 Antibody Product Information

• Immunogen: Recombinant Human TROP2 protein (Catalog#10428-H08H)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 4F4B1B3
• Ig Type: Mouse IgG1
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 / TACSTD2 (rh TROP2 / TACSTD2; Catalog#10428-H08H; NP_002344.2; Met 1-Thr 274) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

TROP2 / TACSTD2 Antibody Usage Guide

• Specificity: Human TROP2 / TACSTD2
• No cross-reactivity in ELISA with
  

  Human E-cad

  Human CD146

  Human CD171

  Human BCAM

  Human VCAM1

  Human ICAM1

  Human cell lysate (293 cell line)

• Flow Cytometry: MCF-7 cells were detached using 1X trypsin, washed, then stained with FITC Mouse anti-human TROP2.
  

  Flow cytometric analysis of anti-human TROP2 on MCF-7 cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TROP2 in WB.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

TROP2 / TACSTD2 Antibody Background

TROP2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed by human carcinomas. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. The cytoplasmic tail of TROP2 possesses potential serine and tyrosine phosphorylation sites and a conserved phosphatidyl-inositol binding consensus sequence. TROP2 transduces an intracellular calcium signal and acts as a cell surface receptor, meanwhile is involved in the regulation of cell- cell adhesion. Accordingly, as a widespread stimulator of human cancer growth and a unique marker and causal factor of metastatic cancer, TROP2 is a possible candidate for diagnosis and molecular target therapy. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.

References

1. Fornaro, M. et al., 1995, Int. J. Cancer. 62: 610-618.
2. Ripani, E. et al., 1998, Int. J. Cancer. 76: 671-676.
3. Linnenbach, A.J. et al., 1993, Mol. Cell. Biol. 13: 1507-1515.
4. Ohmachi, T. et al., 2006, Clin. Cancer. Res. 12: 3057-3063.
5. Tsujikawa, M. et al., 1999, Nat. Genet. 21: 420-423.