|Recombinant Human TROP2 protein (Catalog#10428-H08H)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; Catalog#10428-H08H; NP_002344.2; Met 1-Thr 274). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
|Human TROP2 / TACSTD2|
No cross-reactivity in WB and ELISA with
Human BCAM / CD239
Human VCAM1 / CD106
Human ICAM1 / CD54
Human CD146 / MCAM / MUC18
Human CD171 / NCAML1 / L1CAM
Human Cadherin-1 / E-cadherin / CDH1 / CD324
Human cell lysate (293 cell line)
|ELISA, IHC-P, FCM|
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TROP2. The detection limit for Human TROP2 is 0.039 ng/well.
IHC-P: 5-15 μg/mL
FCM: 0.5-2 μg/Test
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.