|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
TROP2 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate0.5 µL anti-TROP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-TROP2 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 35 kDa
Observed band size: 35 kDa
Anti-TROP2 rabbit polyclonal antibody at 1:500 dilution
Lane A: 293T Membrane LysateLysates/proteins at 20 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:36 kDa
Observed band size:38 kDa
TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-TROP2 Antibody (FITC)||Mouse||Monoclonal||FCM,ICC/IF,IF||10428-MM01-F|
|Anti-TROP2 Antibody (FITC)||Rabbit||Monoclonal||FCM,ICC/IF,IF||10428-R001-F|
|Anti-TROP2 Antibody (PE)||Mouse||Monoclonal||FCM||10428-MM01-P|
|Anti-TROP2 Antibody (APC)||Rabbit||Monoclonal||FCM||10428-R001-A|
|Anti-TROP2 Antibody (PE)||Rabbit||Monoclonal||FCM||10428-R001-P|