Anti-TROP2 Antibody

Cat: 10428-RP02
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Anti-TROP2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-TROP2 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Not React with: Mouse, Rat
Specificity
Human TROP2
Immunogen
Recombinant Human TROP2 protein (Catalog#10428-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; Catalog#10428-H08H; NP_002344.2; Met 1-Thr 274). TROP2 specific IgG was purified by human TROP2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TROP2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IHC-P 1:5000-1:25000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TROP2 Antibody (Rabbit Polyclonal antibody) Images
Human TROP-2/TACSTD2/TACD2 Immunohistochemistry(IHC) 3469
Immunochemical staining of human TROP2 in human rectal cancer with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.
Human TROP-2/TACSTD2/TACD2 Immunohistochemistry(IHC) 3477
Immunochemical staining of human TROP2 in human placenta with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of cytotrophoblast.
Human TROP-2/TACSTD2/TACD2 Immunohistochemistry(IHC) 3481
Immunochemical staining of human TROP2 in human mammary gland with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.
Human TROP-2/TACSTD2/TACD2 Immunoprecipitation(IP) 7984

TROP2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-TROP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TROP2 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Human TROP-2/TACSTD2/TACD2 Western blot (WB) 13216

Anti-TROP2 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:38 kDa

Anti-TROP2 Antibody Alternative Names
Anti-EGP-1 Antibody;Anti-EGP1 Antibody;Anti-GA733-1 Antibody;Anti-GA7331 Antibody;Anti-GP50 Antibody;Anti-M1S1 Antibody;Anti-TACD2 Antibody;Anti-TROP-2 Antibody;Anti-TROP2 Antibody
TROP2 Background Information

TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

Full Name
tumor-associated calcium signal transducer 2
References
  • Ripani E, et al. (1998) Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 76(5): 671-6.
  • Wang J, et al. (2008) Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol Cancer Ther. 7(2): 280-5.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TROP2 Antibody Rabbit Monoclonal ELISA 50922-R064
    Anti-TROP2 Antibody Rabbit Monoclonal ELISA 50922-R065
    Anti-TROP2 Antibody Rabbit Polyclonal ELISA,IHC-P 50922-RP02
    Anti-TROP2 Antibody Rabbit Monoclonal IHC-P 50922-R046
    Anti-TROP2 Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 10428-MM01-F
    Anti-TROP2 Antibody Mouse Monoclonal ELISA(Cap),IHC-P,ICC/IF,IF 10428-MM02
    Anti-TROP2 Antibody (FITC) Rabbit Monoclonal FCM,ICC/IF,IF 10428-R001-F
    Anti-TROP2 Antibody Rabbit Polyclonal ELISA 10428-RP01
    Anti-TROP2 Antibody (PE) Mouse Monoclonal FCM 10428-MM01-P
    Anti-TROP2 Antibody (APC) Rabbit Monoclonal FCM 10428-R001-A
    Anti-TROP2 Antibody (PE) Rabbit Monoclonal FCM 10428-R001-P
    Anti-TROP2 Antibody Rabbit Monoclonal WB,ELISA,IHC-P 10428-R030
    Anti-TROP2 Antibody Rabbit Polyclonal ELISA,IHC-P 10428-T24
    Anti-TROP2 Antibody Mouse Monoclonal ELISA,ELISA(Det),IHC-P,FCM 10428-MM01
    Anti-TROP2 Antibody Rabbit Monoclonal ELISA,FCM,ICC/IF,IF 10428-R001
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