Mouse TRIB3 Gene cDNA Clone / ORF Clone

TRIB3 cDNA Clone Datasheet

TRIB3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_175093.2
• cDNA Size: 1065
• Gene Synonym: Nipk; SINK; Trb3; Ifld2; SKIP3; TRB-3; Trib3
• Source: Mouse

TRIB3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tribbles homolog 3 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 717 C/T not causing the amino acid variation.

TRIB3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TRIB3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TRIB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGAGCTA CACCTCTGGC TGCTTCTGCT GATGTTTCCT GCAGGAAGAA ACCGTTGGAG
61       TTTGATGACA ATATTGATGC CAAGTGTCCA GTCCTAAAAC GAGTGAGAGA TGAGCCTGAG
121      CCCGGACCAC TCCCCAGCCT GCTGCCCCCC AGCCCACCTC CCGCCTCAGA CTTGTCACCT
181      GCTGTGGCCC CTGCAACTCG ACTGGGGCCT TATATCCTTT TGGAACGAGA GCAAGGCAGC
241      TGCAGCTATC GAGCCCTGCA CTGCCCCACA GGCACAGAGT ACACCTGCAA GGTGTACCCT
301      GCCAGCGAGG CCCAGGCGGT GCTGGCACCT TATGCCCGGC TGCCTACCCA CCAGCATGTG
361      GCCCGTCCCA CAGAGGTCCT GCTGGGCTCT CGGCTCCTTT ACATCTTTTT CACGAAGACC
421      CATGGGGACT TGCACAGCCT GGTGCGCAGC CGCCGCGGTA TCCCAGAGTC CGAGGCTGCC
481      GGGCTCTTCC GGCAGATGGC TAGTGCCGTG GCACACTGCC ACAAGCACGG GCTTGTCTTG
541      CGCGACCTCA AGCTGCGTCG CTTTGTCTTC AGCAACTGTG AGAGGACGAA GCTGGTGCTG
601      GAGAACCTGG AAGATGCCTG CGTGATGACT GGATCAGATG ACTCTCTGTG GGACAAGCAT
661      GCGTGCCCTG CCTACGTGGG ACCAGAGATA CTCAGCTCCC GGCCATCCTA CTCTGGTAAA
721      GCGGCTGATG TCTGGAGCCT GGGTGTGGCG CTCTTCACCA TGCTGGCTGG CAGATACCCA
781      TTCCACGACT CTGAGCCAGT CCTGCTCTTT GGCAAGATCC GTAGAGGGAC CTTTGCCCTG
841      CCTGAGGGCC TATCAGCCCC AGCCCGCTGT CTGATCCGCT GTCTCCTCCG CAAGGAACCT
901      TCAGAGCGAC TTGTGGCCCT GGGAATCCTC TTGCATCCCT GGTTGAGAGA GGATCACGGC
961      CGAGTCTCTC CTCCACAGTC TGACCGAAGG GAGATGGACC AGGTGGTCCC AGATGGGCCA
1021     CAGCTGGAGG AGGCTGAGGA AGGGGAGGTG GGGCTGTACG GCTAG

 

Quality Control:cDNA sequencing results is available upon request.