Mouse TRIB2 Gene cDNA Clone / ORF Clone

TRIB2 cDNA Clone Datasheet

TRIB2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_144551.5
• cDNA Size: 1032
• Gene Synonym: TRB2; TRB-2; AW319517; Trib2
• Source: Mouse

TRIB2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tribbles homolog 2 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 786 T/C not causing the amino acid variation.

TRIB2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TRIB2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TRIB2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACATAC ACAGGTCTAC CCCTATCACA ATAGCGAGAT ATGGGAGATC GCGGAACAAA
61       ACCCAGGATT TCGAAGAGCT GTCGTCTATA AGGTCCGCTG AGCCCAGCCA GAGTTTCAGC
121      CCGAACCTTG GCTCTCCGAG CCCGCCCGAG ACTCCGAACT TGTCGCATTG CGTTTCTTGC
181      ATCGGGAAAT ACTTACTGTT GGAGCCTCTG GAGGGAGACC ACGTTTTCCG CGCTGTGCAT
241      CTGCACAGCG GAGAGGAGCT GGTTTGCAAG GTGTTTGAGA TCAGCTGCTA CCAGGAGTCC
301      CTGGCCCCCT GCTTCTGCCT GTCTGCCCAT AGCAACATCA ACCAAATCAC GGAAATCCTC
361      CTGGGAGAGA CCAAAGCCTA TGTGTTCTTT GAGCGAAGCT ATGGAGACAT GCATTCCTTT
421      GTCCGCACTT GTAAGAAGCT GAGGGAGGAG GAGGCAGCCC GACTGTTCTA CCAGATTGCC
481      TCAGCTGTGG CCCATTGCCA CGATGGAGGC CTGGTGCTGC GTGACCTCAA GCTGCGGAAA
541      TTTATCTTCA AGGATGAAGA GAGGACTCGT GTCAAGCTGG AGAGTTTGGA AGACGCTTAC
601      ATTCTCCGGG GTGATGATGA CTCACTCTCT GACAAGCATG GCTGCCCAGC GTATGTCAGC
661      CCAGAGATCT TGAACACCAG CGGCAGTTAT TCGGGCAAGG CAGCGGACGT GTGGAGCCTG
721      GGGGTAATGC TGTACACCAT GTTGGTGGGG CGTTACCCTT TCCATGACAT TGAGCCTAGT
781      TCTCTCTTCA GTAAGATCCG CAGGGGCCAG TTCAACATTC CAGAAACTCT GTCTCCCAAG
841      GCCAAGTGCC TCATCCGAAG CATCCTGCGA CGGGAGCCGT CAGAGCGGCT GACCTCGCAG
901      GAAATTCTGG ACCATCCTTG GTTTTCTACA GATTTTAGTG TCTCAAATTC GGGATTTGGT
961      GCTAAAGAGG CGTGTGACCA GCTGGTGCCA GACGTCAACA TGGAGGAGAA CTTGGACCCT
1021     TTCTTTAACT GA

 

Quality Control:cDNA sequencing results is available upon request.