TRAIL R4 / CD264 / TNFRSF10D Antibody

TRAIL R4 / CD264 / TNFRSF10D Antibody Datasheet

TRAIL R4 / CD264 / TNFRSF10D Antibody Product Information

• Immunogen: Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (Catalog#10413-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2F8B1
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TRAIL R4 / CD264 / TNFRSF10D (rh TRAIL R4 / CD264 / TNFRSF10D; Catalog#10413-H08H; NP_003831.2; Met 1-His 211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

TRAIL R4 / CD264 / TNFRSF10D Antibody Usage Guide

• Specificity: Human TRAIL R4 / CD264 / TNFRSF10D
• No cross-reactivity in ELISA with

  Human TNFRSF10A

  Human TNFRSF10B

  Human TNFa

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TNFRSF10D in WB. Using a DAB detection system, the detection limit for Human TNFRSF10D is approximately 8 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TNFRSF10D. The detection limit for Human TNFRSF10D is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TRAIL R4 / CD264 / TNFRSF10D Antibody Background

TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, are TNF superfamily members, and preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least five different receptors with characteristic cysteine-rich repeats. Human TRAILR4, also known as decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a member of the TNF receptor superfamily and is referred to as TNFRSF10D. TRAILR4 shows a unique expression pattern in human tissues and is particularly abundant in fetal liver and adult testis. This protein is a type 1 transmembrane receptor consisting of an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. Accordingly, TRAILR4 is not capable of activating TRAIL-mediated NF-kappaB or apoptosis pathways, and identified as an antiapoptotic receptor, although it retains one third of a consensus death domain motif in its cytoplasmic domain. It has been demonstrated that TRAILR4 expression is directly regulated by p53 and attenuates cell response to DNA-damaging agents.

References

1. Degli-Esposti, M.A. et al., 1997, Immunity. 7: 813-820.
2. Pan, G. et al., 1998, FEBS. Lett. 424: 41-45.
3. Shivapurkar, N. et al., 2004, Int. J. Cancer. 109: 786-792.
4. Liu, X. et al., 2005, Cancer. Res. 65: 9169-9175.
5. Baetu, T.M. and Hiscott, J. 2002, Cytokine. Growth. Factor. Rev. 13: 199-207.