TRAIL R2 / TNFRSF10B / CD262 Antibody ( PE ) (Cytokine)

TRAIL R2 / TNFRSF10B / CD262 Antibody Datasheet

TRAIL R2 / TNFRSF10B / CD262 Antibody Product Information

• Immunogen: Recombinant Human TRAIL R2 / TNFRSF10B / CD262 protein (Catalog#10465-H08H)
• Reagents: PE-conjugated rabbit monoclonal antibody
• Clone ID: 102
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human TRAIL R2 / TNFRSF10B / CD262 (rh TRAIL R2 / TNFRSF10B / CD262; Catalog#10465-H08H; NP_003833.3; Met 1-Glu 182) and conjugated with PE under optimum conditions, the unreacted PE was removed.

TRAIL R2 / TNFRSF10B / CD262 Antibody Usage Guide

• Specificity: Human TRAIL R2 / TNFRSF10B / CD262
• No cross-reactivity in ELISA with
  

  Human TNFRSF10A / CD261 / APO2

  Human TNFRSF10D / CD264 / DCR2 / TRAILR4

• Flow Cytometry:
  

  Profile of anti-TNFRSF10B (CD262) reactivity on Jurkat cells analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

TRAIL R2 / TNFRSF10B / CD262 Antibody Background

TRAILR2, also known as DR5 and TNFRSF10B, is the fifth death domain-containing member of the TNFR family and a receptor for TRAIL/TNFSF10. TRAILR2 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. This single-pass transmembrane protein contains two cysteine-rich repeat units in its extracellular region, followed by a predicted hydrophobic transmembrane segment and a 209 aa long cytoplasmic tail containing a typical "death domain". Upon ligand binding, the adapter molecule FADD recruits caspase-8 to the activated receptor TNFRSF10B. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases mediating apoptosis. TRAIL-R2 exhibits high structural and functional homology to TRAIL-R1 (DR-4), and TNFRSF10B expression is regulated by the tumor suppressor p53. Further, it is also indicated that the activation of NF-kappa-B can be promoted by TNFRSF10B.

References

1. Henning Walczak, et al., 1997, The EMBO Journal, Vol.16, No.17: 5386-5397.
2. G. Pan, et al.,1997, Science, 277: 815-818.
3. Pascal Schneider, et al., 1997, FEBS Letters, Volume 416, Issue 3: 329-334.
4. Gavin R.Screaton, et al., 1997, Current Biology, Volume 7, Issue 9: 693-696.
5. Sarah G Hymowit, et al., 1999, Molecular Cell, Volume 4, Issue 4: 563-5.
6. Preet M. Chaudhary, et al., 1997, Immunity, Volume 7, Issue 6: 821-830.