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TPSB2 / Tryptase Beta 2 Antibody, Mouse MAb
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TPSB2 / Tryptase Beta 2 Antibody, Mouse MAb PDF Download

Catalog   Size (Price) Quantity In Stock Operation
10505-MM03  

YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

TPSB2 / Tryptase Beta 2 Antibody Datasheet

  Order or Inquire for TPSB2 / Tryptase Beta 2 Antibody product Quality antibodies Antibody production services
  Detection limit is 0.16 ng/well in ELISA
 

TPSB2 / Tryptase Beta 2 Antibody Product Information

Immunogen :

Recombinant Human TPSB2 / Tryptase Beta 2 protein (Catalog#10505-H08H)

Antibody Type : Mouse Monoclonal Antibody ( Mouse mAb Service Platform )

Clone ID :

03

Ig Type :

Mouse IgG1

Formulation : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
Preparation :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TPSB2 / Tryptase Beta 2 (rh TPSB2 / Tryptase Beta 2; Catalog#10505-H08H; NP_077078.5; Met 1-Pro 275). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

TPSB2 / Tryptase Beta 2 Antibody Usage Guide

Specificity :

Human TPSB2 / Tryptase Beta 2

 

No cross-reactivity in ELISA with

Human cell lysate (293 cell line)

Direct ELISA : This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TPSB2. The detection limit for Human TPSB2 is approximately 0.16 ng/well.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TPSB2 / Tryptase Beta 2 Antibody Related Products & Topics

Related Areas:

Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>TPSB2/Tryptase Beta 2

Immunology>>Innate Immunity>>Mast Cell>>TPSB2/Tryptase Beta 2

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
TPSB2/Tryptase Beta 2 Human TPSB2 Protein, Recombinant 10505-H08H

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human TPSB2/Tryptase Beta 2 ELISA TPSB2 / Tryptase Beta 2 Antibody 10505-MM03

TPSB2 / Tryptase Beta 2 Antibody Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1, and fall into two groups, α and β. β-tryptases appear to be the main isoenzymes expressed in mast cells, whereas α-tryptases predominate in basophils. Tryptase is unique in two respects: it is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known endogenous proteinase inhibitors because of the unique arrangement of the active sites. Additionally, tryptase family genes have an intron immediately upstream of the initiator codon which separates the transcription initiation site from protein coding sequence, and this feature is characteristic of tryptases. β-tryptases existing in three isoforms (β1,β2,β3) are released in secretory granules, and have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. It has been reported that β-tryptase selectively cleaves ASM-derived eotaxin and RANTES and abrogates their chemotactic activities.

References

  1. Miller, J.S. et al., 1990, J. Clin. Invest. 86: 864-870.
  2. Pereira, P.J. et al., 1998, Nature. 392: 306-311.
  3. Pallaoro, M.et al., 1999, J. Biol. Chem. 274: 3355-3362.
  4. Pang, L. et al., 2006, J. Immunol. 176: 3788-3795.
 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"