Anti-TPSB2 Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Recombinant Human TPSB2 / Tryptase Beta 2 protein (Catalog#10505-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TPSB2 / Tryptase Beta 2 (rh TPSB2 / Tryptase Beta 2; Catalog#10505-H08H; NP_077078.5; Met 1-Pro 275). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #03
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-TPSB2 Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-TPSB2 Antibody Alternative Names
Anti-TPS2 Antibody;Anti-tryptaseB Antibody;Anti-tryptaseC Antibody
TPSB2 Background Information
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1, and fall into two groups, α and β. β-tryptases appear to be the main isoenzymes expressed in mast cells, whereas α-tryptases predominate in basophils. Tryptase is unique in two respects: it is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known endogenous proteinase inhibitors because of the unique arrangement of the active sites. Additionally, tryptase family genes have an intron immediately upstream of the initiator codon which separates the transcription initiation site from protein coding sequence, and this feature is characteristic of tryptases. β-tryptases existing in three isoforms (β1,β2,β3) are released in secretory granules, and have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. It has been reported that β-tryptase selectively cleaves ASM-derived eotaxin and RANTES and abrogates their chemotactic activities.
tryptase beta 2 (gene/pseudogene)
Miller, J.S. et al., 1990, J. Clin. Invest. 86: 864-870. Pereira, P.J. et al., 1998, Nature. 392: 306-311. Pallaoro, M.et al., 1999, J. Biol. Chem. 274: 3355-3362. Pang, L. et al., 2006, J. Immunol. 176: 3788-3795.