TPSB2 / Tryptase Beta 2 Antibody

TPSB2 / Tryptase Beta 2 Antibody Datasheet

TPSB2 / Tryptase Beta 2 Antibody Product Information

• Immunogen: Recombinant Human TPSB2 / Tryptase Beta 2 protein (Catalog#10505-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 03
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TPSB2 / Tryptase Beta 2 (rh TPSB2 / Tryptase Beta 2; Catalog#10505-H08H; NP_077078.5; Met 1-Pro 275). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

TPSB2 / Tryptase Beta 2 Antibody Usage Guide

• Specificity: Human TPSB2 / Tryptase Beta 2
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TPSB2. The detection limit for Human TPSB2 is approximately 0.16 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TPSB2 / Tryptase Beta 2 Antibody Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1, and fall into two groups, α and β. β-tryptases appear to be the main isoenzymes expressed in mast cells, whereas α-tryptases predominate in basophils. Tryptase is unique in two respects: it is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known endogenous proteinase inhibitors because of the unique arrangement of the active sites. Additionally, tryptase family genes have an intron immediately upstream of the initiator codon which separates the transcription initiation site from protein coding sequence, and this feature is characteristic of tryptases. β-tryptases existing in three isoforms (β1,β2,β3) are released in secretory granules, and have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. It has been reported that β-tryptase selectively cleaves ASM-derived eotaxin and RANTES and abrogates their chemotactic activities.

References

1. Miller, J.S. et al., 1990, J. Clin. Invest. 86: 864-870.
2. Pereira, P.J. et al., 1998, Nature. 392: 306-311.
3. Pallaoro, M.et al., 1999, J. Biol. Chem. 274: 3355-3362.
4. Pang, L. et al., 2006, J. Immunol. 176: 3788-3795.