Human Thyroid peroxidase / TPO Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13194-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TPO cDNA Clone Datasheet

TPO cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000547.5
cDNA Size:	2802
Gene Synonym:	MSA; TPX; TDH2A
Source:	Human

TPO cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens thyroid peroxidase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 769 G/T, 2173 A/C, 2540 T/C resulting in the amino acid Ala substitution by Ser, Thr substitution by Pro, Val substitution by Ala and 1998 C/T, 2145 C/T not causing the amino acid variation.

TPO cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TPO cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TPO cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAGCGC TCGCTGTGCT GTCTGTCACG CTGGTTATGG CCTGCACAGA AGCCTTCTTC
61       CCCTTCATCT CGAGAGGGAA AGAACTCCTT TGGGGAAAGC CTGAGGAGTC TCGTGTCTCT
121      AGCGTCTTGG AGGAAAGCAA GCGCCTGGTG GACACCGCCA TGTACGCCAC GATGCAGAGA
181      AACCTCAAGA AAAGAGGAAT CCTTTCTCCA GCTCAGCTTC TGTCTTTTTC CAAACTTCCT
241      GAGCCAACAA GCGGAGTGAT TGCCCGAGCA GCAGAGATAA TGGAAACATC AATACAAGCG
301      ATGAAAAGAA AAGTCAACCT GAAAACTCAA CAATCACAGC ATCCAACGGA TGCTTTATCA
361      GAAGATCTGC TGAGCATCAT TGCAAACATG TCTGGATGTC TCCCTTACAT GCTGCCCCCA
421      AAATGCCCAA ACACTTGCCT GGCGAACAAA TACAGGCCCA TCACAGGAGC TTGCAACAAC
481      AGAGACCACC CCAGATGGGG CGCCTCCAAC ACGGCCCTGG CACGATGGCT CCCTCCAGTC
541      TATGAGGACG GCTTCAGTCA GCCCCGAGGC TGGAACCCCG GCTTCTTGTA CAACGGGTTC
601      CCACTGCCCC CGGTCCGGGA GGTGACAAGA CATGTCATTC AAGTTTCAAA TGAGGTTGTC
661      ACAGATGATG ACCGCTATTC TGACCTCCTG ATGGCATGGG GACAATACAT CGACCACGAC
721      ATCGCGTTCA CACCACAGAG CACCAGCAAA GCTGCCTTCG GGGGAGGGTC TGACTGCCAG
781      ATGACTTGTG AGAACCAAAA CCCATGTTTT CCCATACAAC TCCCGGAGGA GGCCCGGCCG
841      GCCGCGGGCA CCGCCTGTCT GCCCTTCTAC CGCTCTTCGG CCGCCTGCGG CACCGGGGAC
901      CAAGGCGCGC TCTTTGGGAA CCTGTCCACG GCCAACCCGC GGCAGCAGAT GAACGGGTTG
961      ACCTCGTTCC TGGACGCGTC CACCGTGTAT GGCAGCTCCC CGGCCCTAGA GAGGCAGCTG
1021     CGGAACTGGA CCAGTGCCGA AGGGCTGCTC CGCGTCCACG CGCGCCTCCG GGACTCCGGC
1081     CGCGCCTACC TGCCCTTCGT GCCGCCACGC GCGCCTGCGG CCTGTGCGCC CGAGCCCGGC
1141     ATCCCCGGAG AGACCCGCGG GCCCTGCTTC CTGGCCGGAG ACGGCCGCGC CAGCGAGGTC
1201     CCCTCCCTGA CGGCACTGCA CACGCTGTGG CTGCGCGAGC ACAACCGCCT GGCCGCGGCG
1261     CTCAAGGCCC TCAATGCGCA CTGGAGCGCG GACGCCGTGT ACCAGGAGGC GCGCAAGGTC
1321     GTGGGCGCTC TGCACCAGAT CATCACCCTG AGGGATTACA TCCCCAGGAT CCTGGGACCC
1381     GAGGCCTTCC AGCAGTACGT GGGTCCCTAT GAAGGCTATG ACTCCACCGC CAACCCCACT
1441     GTGTCCAACG TGTTCTCCAC AGCCGCCTTC CGCTTCGGCC ATGCCACGAT CCACCCGCTG
1501     GTGAGGAGGC TGGACGCCAG CTTCCAGGAG CACCCCGACC TGCCCGGGCT GTGGCTGCAC
1561     CAGGCTTTCT TCAGCCCATG GACATTACTC CGTGGAGGTG GTTTGGACCC ACTAATACGA
1621     GGCCTTCTTG CAAGACCAGC CAAACTGCAG GTGCAGGATC AGCTGATGAA CGAGGAGCTG
1681     ACGGAAAGGC TCTTTGTGCT GTCCAATTCC AGCACCTTGG ATCTGGCGTC CATCAACCTG
1741     CAGAGGGGCC GGGACCACGG GCTGCCAGGT TACAATGAGT GGAGGGAGTT CTGCGGCCTG
1801     CCTCGCCTGG AGACCCCCGC TGACCTGAGC ACAGCCATCG CCAGCAGGAG CGTGGCCGAC
1861     AAGATCCTGG ACTTGTACAA GCATCCTGAC AACATCGATG TCTGGCTGGG AGGCTTAGCT
1921     GAAAACTTCC TCCCCAGGGC TCGGACAGGG CCCCTGTTTG CCTGTCTCAT TGGGAAGCAG
1981     ATGAAGGCTC TGCGGGATGG TGACTGGTTT TGGTGGGAGA ACAGCCACGT CTTCACGGAT
2041     GCACAGAGGC GTGAGCTGGA GAAGCACTCC CTGTCTCGGG TCATCTGTGA CAACACTGGC
2101     CTCACCAGGG TGCCCATGGA TGCCTTCCAA GTCGGCAAAT TCCCTGAAGA CTTTGAGTCT
2161     TGTGACAGCA TCCCTGGCAT GAACCTGGAG GCCTGGAGGG AAACCTTTCC TCAAGACGAC
2221     AAGTGTGGCT TCCCAGAGAG CGTGGAGAAT GGGGACTTTG TGCACTGTGA GGAGTCTGGG
2281     AGGCGCGTGC TGGTGTATTC CTGCCGGCAC GGGTATGAGC TCCAAGGCCG GGAGCAGCTC
2341     ACTTGCACCC AGGAAGGATG GGATTTCCAG CCTCCCCTCT GCAAAGATGT GAACGAGTGT
2401     GCAGACGGTG CCCACCCCCC CTGCCACGCC TCTGCGAGGT GCAGAAACAC CAAAGGCGGC
2461     TTCCAGTGTC TCTGCGCGGA CCCCTACGAG TTAGGAGACG ATGGGAGAAC CTGCGTAGAC
2521     TCCGGGAGGC TCCCTCGGGC GACTTGGATC TCCATGTCGC TGGCTGCTCT GCTGATCGGA
2581     GGCTTCGCAG GTCTCACCTC GACGGTGATT TGCAGGTGGA CACGCACTGG CACTAAATCC
2641     ACACTGCCCA TCTCGGAGAC AGGCGGAGGA ACTCCCGAGC TGAGATGCGG AAAGCACCAG
2701     GCCGTAGGGA CCTCACCGCA GCGGGCCGCA GCTCAGGACT CGGAGCAGGA GAGTGCTGGG
2761     ATGGAAGGCC GGGATACTCA CAGGCTGCCG AGAGCCCTCT GA

Quality Control:cDNA sequencing results is available upon request.