Human TNMD Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13740-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TNMD cDNA Clone Datasheet

TNMD cDNA Clone Product Information

Gene Bank Ref.ID:	NM_022144.2
cDNA Size:	954
Gene Synonym:	TEM; CHM1L; BRICD4; TNMD
Source:	Human

TNMD cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens tenomodulin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 306 G/A not causing the amino acid variation.

TNMD cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNMD cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TNMD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAAAGA ATCCTCCAGA GAATTGTGAA GACTGTCACA TTCTAAATGC AGAAGCTTTT
61       AAATCCAAGA AAATATGTAA ATCACTTAAG ATTTGTGGAC TGGTGTTTGG TATCCTGGCC
121      CTAACTCTAA TTGTCCTGTT TTGGGGGAGC AAGCACTTCT GGCCGGAGGT ACCCAAAAAA
181      GCCTATGACA TGGAGCACAC TTTCTACAGC AATGGAGAGA AGAAGAAGAT TTACATGGAA
241      ATTGATCCTG TGACCAGAAC TGAAATATTC AGAAGCGGAA ATGGCACTGA TGAAACATTG
301      GAAGTACACG ACTTTAAAAA CGGATACACT GGCATCTACT TCGTGGGTCT TCAAAAATGT
361      TTTATCAAAA CTCAGATTAA AGTGATTCCT GAATTTTCTG AACCAGAAGA GGAAATAGAT
421      GAGAATGAAG AAATTACCAC AACTTTCTTT GAACAGTCAG TGATTTGGGT CCCAGCAGAA
481      AAGCCTATTG AAAACCGAGA TTTTCTTAAA AATTCCAAAA TTCTGGAGAT TTGTGATAAC
541      GTGACCATGT ATTGGATCAA TCCCACTCTA ATATCAGTTT CTGAGTTACA AGACTTTGAG
601      GAGGAGGGAG AAGATCTTCA CTTTCCTGCC AACGAAAAAA AAGGGATTGA ACAAAATGAA
661      CAGTGGGTGG TCCCTCAAGT GAAAGTAGAG AAGACCCGTC ACGCCAGACA AGCAAGTGAG
721      GAAGAACTTC CAATAAATGA CTATACTGAA AATGGAATAG AATTTGATCC CATGCTGGAT
781      GAGAGAGGTT ATTGTTGTAT TTACTGCCGT CGAGGCAACC GCTATTGCCG CCGCGTCTGT
841      GAACCTTTAC TAGGCTACTA CCCATATCCA TACTGCTACC AAGGAGGACG AGTCATCTGT
901      CGTGTCATCA TGCCTTGTAA CTGGTGGGTG GCCCGCATGC TGGGGAGGGT CTAA

Quality Control:cDNA sequencing results is available upon request.