Mouse LIGHT/TNFSF14 Gene cDNA Clone / ORF Clone

Tnfsf14 cDNA Clone Datasheet

Tnfsf14 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019418.2
• cDNA Size: 720
• Gene Synonym: LTg; HVEML; LIGHT; Ly113; HVEM-L; Tnfsf14
• Source: Mouse

Tnfsf14 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tumor necrosis factor (ligand) superfamily, member 14 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

Tnfsf14 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Tnfsf14 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

Tnfsf14 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGTG TGGTACAGCC TTCAGTGTTT GTGGTGGATG GACAGACGGA CATCCCATTC
61       AGGCGGCTGG AACAGAACCA CCGGAGACGG CGCTGTGGCA CTGTCCAGGT CAGCCTGGCC
121      CTGGTGCTGC TGCTAGGTGC TGGGCTGGCC ACTCAGGGCT GGTTTCTCCT GAGACTGCAT
181      CAACGTCTTG GAGACATAGT AGCTCATCTG CCAGATGGAG GCAAAGGCTC CTGGGAGAAG
241      CTGATACAAG ATCAACGATC TCACCAGGCC AACCCAGCAG CACATCTTAC AGGAGCCAAC
301      GCCAGCTTGA TAGGTATTGG TGGACCTCTG TTATGGGAGA CACGACTTGG CCTGGCCTTC
361      TTGAGGGGCT TGACGTATCA TGATGGGGCC CTGGTGACCA TGGAGCCCGG TTACTACTAT
421      GTGTACTCCA AAGTGCAGCT GAGCGGCGTG GGCTGCCCCC AGGGGCTGGC CAATGGCCTC
481      CCCATCACCC ATGGACTATA CAAGCGCACA TCCCGCTACC CGAAGGAGTT AGAACTGCTG
541      GTCAGTCGGC GGTCACCCTG TGGCCGGGCC AACAGCTCCC GAGTCTGGTG GGACAGCAGC
601      TTCCTGGGCG GCGTGGTACA TCTGGAGGCT GGGGAAGAGG TGGTGGTCCG CGTGCCTGGA
661      AACCGCCTGG TCAGACCACG TGACGGCACC AGGTCCTATT TCGGAGCTTT CATGGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.