Cynomolgus monkey TNFSF13 Gene cDNA Clone / ORF Clone (Cytokine)

TNFSF13 cDNA Clone Datasheet

TNFSF13 cDNA Clone Product Information

• cDNA Size: 753
• Gene Synonym: TNFSF13
• Source: Cynomolgus monkey

TNFSF13 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) tumor necrosis factor (ligand) superfamily, member 13 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 352 G/C resulting in the amino acid Val substitution by Leu.
  Please check the sequence information before order.

TNFSF13 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFSF13 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TNFSF13 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCAGCCT CATCTCTTTT CTCGCTAGCC CCCAAAGGGC CTCCAGGCAA CATGGGGGTT
61       CCGGTCAGAG AGCCGGCACT CTCAGTTGCC CTCTGGTTGA GTTGGGGTGC AGCTCTGGGG
121      GCTGTGGCTT GTGCCATGGC TCTGCTGACC CAACAGACAG AGCTGCAGAG CCTCAGGAGA
181      GAGGTGAGCC GGCTGCAGAG GACAGGAGGG CCCTCCCAGA AGGCAGAAGG GTATCCCTGG
241      CAGAGTCTCC CGGAGCAGAG CTCCGATGCC CCGGAAGCCT GGGAGAATGG GGAGAGATCC
301      CGGAAAAGGA GAGCAGTGCT CACCCAAAAA CAGAAGAAGC AGCACTCCGT CCTGCACCTG
361      GTTCCCATTA ACGCCACCTC CAAGGATGAC TCCGATGTGA CCGAGGTGAT GTGGCAACCA
421      GCTCTTAGGC GTGGGAGAGG CCTACAGGCC CAAGGATATG GTGTCCGAAT CCGGGATGCT
481      GGAGTTTATC TGCTGTATAG CCAGGTCCTG TTTCAAGACG TGACTTTCAC CATGGGTCAG
541      GTGGTGTCTC GAGAAGGCCA AGGAAAGCAA GAGACTCTAT TTCGATGTAT AAGAAGTATG
601      CCCTCCCACC CGGACCGGGC CTACAACAGC TGCTATAGCG CAGGTGTCTT CCATTTACAC
661      CAAGGGGATA TTCTAAGTGT CATAATTCCC CGGGCAAGGG CGAAACTTAA CCTCTCTCCA
721      CATGGAACCT TCCTGGGGTT TGTGAAACTG TGA

 

Quality Control:cDNA sequencing results is available upon request.