Cynomolgus monkey TNFSF12 Gene cDNA Clone / ORF Clone (Cytokine)

TNFSF12 cDNA Clone Datasheet

TNFSF12 cDNA Clone Product Information

• cDNA Size: 750
• Gene Synonym: TNFSF12
• Source: Cynomolgus monkey

TNFSF12 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) tumor necrosis factor (ligand) superfamily, member 12 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 372 G/A and 666 G/C not causing the amino acid variation.
  Please check the sequence information before order.

TNFSF12 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFSF12 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TNFSF12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGCCC GTCGGAGCCA GAGGCGGAGG GGGCGCCGGG GGGAGCCGGG CACCGCCCTG
61       CTGGTCCCGC TCGCGCTGGG CCTGGGCCTG GCGCTGGCCT GCCTCGGCCT CCTGCTGGCC
121      GTGGTCAGCC TGGGGAGCCG GGCATCGCTG TCCGCCCAGG AGCCTGCCCA GGAGGAGCTG
181      GTGGCAGAGG AAGACCAGGA CCCATCGGAA CTGAATCCCC AGGCAGAAGA AAGTCGGGAT
241      CCTGTGCCTT TCCTGAACCG ACTAGTTCGG CCTCGCAGAA GTGCACCTAA AGGCCGGAAA
301      ACACGGGCTC GAAGAGCGAT CGCAGCCCAT TATGAAGTTC ATCCACGACC TGGACAGGAT
361      GGAGCGCAGG CAGGTGTGGA CGGGACAGTG AGTGGCTGGG AGGAAGCCAG AATCAACAGC
421      TCCAGCCCTC TGCGCTACAA CCGCCAGATC GGGGAGTTCA TAGTCACCCG GGCTGGGCTC
481      TACTACCTGT ACTGTCAGGT GCACTTTGAC GAGGGGAAGG CTGTCTACCT GAAGCTGGAC
541      TTGCTGGTGG ATGGCGTGCT GGCCCTGCGC TGCCTGGAGG AATTCTCAGC CACTGCAGCG
601      AGTTCCCTCG GGCCCCAGCT TCGCCTCTGC CAGGTGTCTG GGCTGTTGGC TCTGCGGCCA
661      GGGTCCTCCC TGCGAATCCG CACCCTCCCC TGGGCCCATC TCAAGGCTGC CCCCTTCCTC
721      ACCTACTTCG GACTCTTCCA GGTTCACTGA

 

Quality Control:cDNA sequencing results is available upon request.