TNFSF10 / TRAIL / APO-2L Protein & Antibody (CD253 Protein)

Tumor Necrosis Factor (ligand) Superfamily, member 10 / TNF-Related Apoptosis-Inducing Ligand / Apo-2 Ligand (Cluster of Differentiation 253)

TNFSF10 / TRAIL / APO-2L Products

TNFSF10 / TRAIL / APO-2L Protein, Recombinant

Molecule	Species	Description	Cat. No
TNFSF10/TRAIL/APO-2L/CD253	Human	TNFSF10/TRAIL/APO-2L/CD253 Protein, Recombinant, Native	10409-HNAE
TNFSF10/TRAIL/APO-2L/CD253	Human	TNFSF10/TRAIL/APO-2L/CD253 Protein, Recombinan	10409-H07E
TNFSF10/TRAIL/APO-2L/CD253	Mouse	TNFSF10/TRAIL/APO-2L/CD253 (aa 118-291) Protein, Recombinan	50166-M07E

  10409-H07E:

1. Immobilized human TNFSF10 at 10 µg/ml (100 µl/well) can bind human TNFRSF10A with a linear range of 0.625-20 ng/ml. Measured by its binding ability in a functional ELISA.

2. Immobilized human TNFSF10 at 10 µg/ml (100 µl/well) can bind human TNFRSF10B with a linear range of 2.5-40 ng/ml. Measured by its binding ability in a functional ELISA.

3.Immobilized human TNFRSF10D at 10 µg/ml (100 µl/well) can bind biotinylated TNFSF10 with a linear range of 0.625-20 ng/ml. Measured by its binding ability in a functional ELISA.

  10409-HNAE:

1. The ED₅₀ for this effect is typically 4-8 ng/ml. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

2. The ED₅₀ for this effect is typically 0.2-0.8 ng/ml. Measured in a cytotoxicity assay using NCI-H460 cells in the presence of the metabolic inhibitor actinomycin D.

TNFSF10 / TRAIL / APO-2L Antibody

Molecule	Application	Description	Cat. No
Human TNFSF10/TRAIL/APO-2L/CD253	WB,ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10409-RP02

TNFSF10 / TRAIL / APO-2L cDNA Clone

Molecule	Species	Description	Cat. No
TNFSF10/TRAIL/APO-2L/CD253	Human	Homo sapiens TNFSF10/TRAIL/APO-2L/CD253 cDNA Clone	HG10409-M
TNFSF10/TRAIL/APO-2L/CD253	Mouse	Mus musculus TNFSF10/TRAIL/APO-2L cDNA Clone	MG50166-M
TNFSF10/TRAIL/APO-2L/CD253	Rat	Rat TNFSF10/TRAIL/APO-2L cDNA Clone	RG80152-G
TNFSF10/TRAIL/APO-2L/CD253	Cynomolgus	Cynomolgus monkey TNFSF10 cDNA Clone / ORF Clone	KG90098-G

TNFSF10 / TRAIL / APO-2L Related Areas

Immunology>>Cluster of Differentiation>>Natural Killer (NK) Cell CD Antigen>>TNFSF10/TRAIL/APO-2L/CD253

Immunology>>Innate Immunity>>Natural Killer Cell (NK Cell)>>TNFSF10/TRAIL/APO-2L/CD253

Immunology>>Cytokine & Receptor>>TNF Superfamily>>TNFSF10/TRAIL/APO-2L/CD253

TNFSF10 / TRAIL / APO-2L Alternative Names

TNFSF10, TRAIL, APO2L, Apo-2L, CD253, TL2 [Homo sapiens]

Tnfsf10, Trail, A330042I21Rik, AI448571, APO-2L, Ly81, TL2 [Mus musculus]

TNFSF10 / TRAIL / APO-2L Background

Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10 / Apo-2L / CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10 / TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10 / CD253 is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10 / Apo-2L / CD253 / TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A / TRAILR1, TNFRSF10B / TRAILR2, TNFRSF10C / TRAILR3, TNFRSF10D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10 / TRAIL may be modulated by binding to the decoy receptors TNFRSF10C / TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

TNFSF10 / TRAIL / APO-2L Related Studies

1. Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
2. Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
3. Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.