Cynomolgus TNFRSF5 / CD40 Protein (His Tag) (Cytokine)

CD40 molecule, TNF receptor superfamily member 5 Protein Datasheet

TNFRSF5 / CD40 Protein Product Information

• Synonym: CD40
• Protein Construction: A DNA sequence encoding the cynomolgus CD40 (Met 1- Arg193) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

TNFRSF5 / CD40 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 21
• Molecular Mass: The recombinant cynomolgus CD40 comprises 184 amino acids and has a calculated molecular mass of 20.7 KDa. The apparent molecular mass of the protein is approximately 31 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TNFRSF5 / CD40 protein

TNFRSF5 / CD40 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFRSF5 / CD40 Protein Description

CD40, also known as Tumor necrosis factor receptor superfamily member 5 ( TNFRSF5 ), is a member of the TNF-receptor superfamily. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The binding of CD154 ( CD40L ) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. AT-hook transcription factor AKNA is thought to coordinately regulate the expression of CD40 and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand CD154 is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. In the macrophage, the secondary signal is CD40L on the T cell which binds CD40 on the macrophage cell surface. As a result, the macrophage expresses more CD40 and TNF receptors on its surface which helps increase the level of activation.

References

1. McWhirter SM. et al., 1999, Proc Natl Acad Sci. 96 (15): 8408-13.
2. Tsukamoto N. et al., 1999, Proc Natl Acad Sci. 96 (4): 1234-9.
3. Ni CZ. et al., 2000, Proc. Natl Acad Sci. 97 (19): 10395-9.
4. Pype S. et al., 2000, J Biol Chem. 275 (24): 18586-93.
5. Contin C. et al., 2004, Néphrologie. 25 (4): 119-26.