Cynomolgus TNFRSF1B Protein (His Tag) (Cytokine)

Tumor necrosis factor receptor superfamily, member 1B Protein Datasheet

TNFRSF1B Protein Product Information

• Synonym: TNFRSF1B
• Protein Construction: A DNA sequence encoding the cynomolgus TNFRSF1B (F7EAE4) (Met1- Asp257) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

TNFRSF1B Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 23
• Molecular Mass: The recombinant cynomolgus TNFRSF1B comprises 246 amino acids and has a calculated molecular mass of 26.5 KDa. The apparent molecular mass of it is approximately 38 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TNFRSF1B protein

TNFRSF1B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFRSF1B Protein Description

Tumor necrosis factor receptor superfamily member 1B, also known as TNFRII, TNFRSF1B, and CD120b, is a member to the TNFR (tumor necrosis factor receptor) superfamily characterized by cysteine-rich extracellular domains. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage–pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.

References

1. Philippe D. et al., 2002, Arthritis Care & Research. 46 (8): 2039-44.
2. Wassink T H. et al., 2000, Molecular psychiatry. 5 (6): 678-82.
3. Alicia SC. et al., 2005, Molecular and Cellular Biology. 25 (11): 4716-26.
4. Ziegenhagen MW. et al., 2000, Journal of Internal Medicine. 248 (1): 33-41.
5. Iris Shai. et al., 2005, Diabetes Care. 28 (6): 1376-82.