Rat TNFRSF1A Gene cDNA Clone / ORF Clone

TNFRSF1A cDNA Clone Datasheet

TNFRSF1A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013091.1
• cDNA Size: 1386
• Gene Synonym: Tnfr1, MGC105478, Tnfrsf1a, Tnfr-1
• Source: Rat

TNFRSF1A cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus tumor necrosis factor receptor superfamily, member 1a DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 688 A/G resulting in the amino acid ile substitution by val and 72 A/G not causing the amino acid variation.

TNFRSF1A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFRSF1A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TNFRSF1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTCTCC CCATCGTGCC TGGCCTGCTG CTGTCACTGG TGCTCCTGGC TCTGCTGATG
61       GGGATACACC CGTCAGGGGT CACCGGACTG GTTCCTTCTC TTGGTGACCG GGAGAAGAGG
121      GATAATTTGT GTCCCCAGGG AAAGTATGCC CATCCAAAGA ATAATTCCAT CTGCTGCACC
181      AAGTGCCACA AAGGAACCTA CTTGGTGAGT GACTGTCCAA GCCCAGGGCA GGAAACAGTC
241      TGCGAGGTGT GTGATAAAGG CACCTTTACA GCTTCGCAGA ACCACGTCAG ACAGTGTCTC
301      AGTTGCAAGA CATGTCGGAA AGAAATGTTC CAGGTGGAGA TTTCTCCTTG CAAAGCTGAC
361      ATGGACACCG TGTGTGGCTG CAAGAAGAAC CAATTCCAGC GCTACCTGAG TGAGACGCAT
421      TTCCAGTGTG TGGACTGCAG CCCCTGCTTC AATGGCACCG TGACAATCCC CTGTAAGGAG
481      AAACAGAACA CCGTGTGTAA CTGCCACGCA GGATTCTTTC TAAGCGGAAA TGAGTGCACC
541      CCTTGCAGCC ACTGCAAGAA AAATCAGGAA TGTATGAAGC TGTGCCTACC TCCAGTTGCA
601      AATGTCACAA ACCCCCAGGA CTCAGGTACT GCCGTGCTGT TGCCTCTGGT TATCTTCCTA
661      GGTCTTTGCC TTTTATTCTT TATCTGCGTC AGTCTACTGT GCCGATATCC CCAGTGGAGG
721      CCCAGGGTCT ACTCCATCAT TTGTAGGGAT TCAGCTCCTG TCAAAGAGGT GGAGGGTGAA
781      GGAATTGTTA CTAAGCCCCT AACTCCAGCC TCTATCCCAG CCTTCAGCCC CAACCCCGGC
841      TTCAACCCCA CTCTGGGCTT CAGCACCACC CCACGCTTCA GTCATCCTGT CTCCAGTACC
901      CCCATCAGCC CCGTCTTCGG TCCTAGTAAC TGGCACAACT TCGTGCCACC TGTAAGAGAG
961      GTGGTCCCAA CCCAGGGTGC TGACCCTCTC CTCTACGGAT CCCTCAACCC TGTGCCAATC
1021     CCCGCCCCTG TTCGGAAATG GGAAGACGTC GTCGCGGCCC AGCCACAACG GCTTGACACT
1081     GCAGACCCTG CGATGCTGTA TGCTGTGGTG GATGGCGTGC CTCCGACACG CTGGAAGGAG
1141     TTCATGCGGC TCCTGGGGCT GAGCGAGCAC GAGATCGAGC GGCTGGAGCT GCAGAACGGG
1201     CGTTGCCTCC GCGAGGCTCA TTACAGCATG CTGGAAGCCT GGCGGCGCCG CACACCGCGA
1261     CACGAGGCCA CGCTGGACGT AGTGGGCCGC GTGCTTTGCG ACATGAACCT GCGTGGCTGC
1321     CTGGAGAACA TCCGCGAGAC TCTAGAAAGC CCTGCCCACT CGTCCACGAC CCACCTCCCG
1381     CGATAA

 

Quality Control:cDNA sequencing results is available upon request.