TNFRSF19 / TROY Protein (Cytokine)

Tumor necrosis factor receptor superfamily, member 19 Protein Datasheet

TNFRSF19 / TROY Protein Product Information

• Synonym: TNFRSF19, TAJ, TROY
• Protein Construction: A DNA sequence encoding the human TNFRSF19 (Q9NS68-2) (Met1-Leu170) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

TNFRSF19 / TROY Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 30
• Molecular Mass: The recombinant human TNFRSF19/Fc is a disulfide-linked homodimer. The reduced monomer comprises 382 amino acids and has a predicted molecular mass of 42.5 kDa. The apparent molecular mass of the protein is approximately 52-57 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TNFRSF19 / TROY protein

TNFRSF19 / TROY Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFRSF19 / TROY Protein Description

Tumor necrosis factor receptor superfamily member 19, also known as, Toxicity and JNK inducer, TNFRSF19, TRADE and Troy, is a member of the TNF-receptor superfamily and contains 3 TNFR-Cys repeats. TNFRSF19 is highly expressed in adult brain, and in embryos from day 11-17, but not earlier. It is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, TNFRSF19 is mainly expressed in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. TNFRSF19 has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TNFRSF19 is the first TNFRSF member that is identified as a biomarker for melanoma. TNFRSF19 also presents a potentially novel cell surface signaling target for inhibitors, cell and/or antibody-based immunotherapies.

References

1. Hu S. et al., 1999, Genomics. 62: 103-7.
2. Eby MT. et al., 2000, J Biol Chem. 275: 15336-42.
3. Kojima T. et al., 2000, J Biol Chem. 275: 20742-7.
4. Spanjaard RA. et al., 2007, Int J Cancer. 120 (6): 1304-10.