|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) tumor necrosis factor receptor superfamily, member 14 DNA.|
|Identical with NM_001039190.1 [ Macaca mulatta (Rhesus monkey) ]: 674 G/A resulting in the amino acid Cys substitution by Tyr and 58 T/A，Phe/Ile，345 G/A not causing the amino acid variation. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey TNFRSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90109-G-F|
|Cynomolgus monkey TNFRSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90109-G-H|
|Cynomolgus monkey TNFRSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90109-G-M|
|Cynomolgus monkey TNFRSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90109-G-N|
|Cynomolgus monkey TNFRSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90109-G-Y|
|Product name||Product name|
Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.