+86-400-890-9989
Product Catalog
> ELISA > ELISA Pair Set > TNFRSF10D / TRAIL R4 ELISA Pair Set, Human ELISA Pair Set TNFRSF10D / TRAIL R4 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10413 |
|
YES | 
|
    |
|
Human TNFRSF10D / TRAIL R4 ELISA Pair Set
 |
Order or Inquire for TNFRSF10D / TRAIL R4 ELISA Pair Set |
|
|
High affinity Human TNFRSF10D / TRAIL R4 ELISA Pair Set |
|
|
Detection limit - 156.25 pg/ml |
|
|
Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
|
|
|
|
|
No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
|
Substrate solution was not added |
Add substrate solution and continue |
|
|
Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
|
|
Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
|
Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
|
|
Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
|
|
Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
|
|
Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
|
Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
|
|
High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
|
Increase cycles of washes and soaking time between washes |
||
|
TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
|
|
Materials were contaminated |
Use clean plates, tubes and pipettes tips |
|
|
Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
|
The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human TNFRSF10D / TRAIL R4 ELISA Pair Set Related Products & Topics
Related Areas:
Immunology>>Cytokine & Receptor>>TNF Superfamily>>TRAIL R4/TNFRSF10D/CD264
Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>TRAIL R4/TNFRSF10D/CD264
Immunology>>Cluster of Differentiation>>Granulocyte CD Antigen>>Neutrophils>>TRAIL R4/TNFRSF10D/CD264
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| TRAIL R4/TNFRSF10D/CD264 | Human | TRAIL R4/TNFESF10D/CD264/Fc Protein, Recombinant |
10413-H03H |
| TRAIL R4/TNFRSF10D/CD264 | Human | TRAIL R4/TNFESF10D/CD264 Protein, Recombinant |
10413-H08H |
Antibodies:
Background
TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, are TNF superfamily members, and preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least five different receptors with characteristic cysteine-rich repeats. Human TRAILR4, also known as decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a member of the TNF receptor superfamily and is referred to as TNFRSF10D. TRAILR4 shows a unique expression pattern in human tissues and is particularly abundant in fetal liver and adult testis. This protein is a type 1 transmembrane receptor consisting of an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. Accordingly, TRAILR4 is not capable of activating TRAIL-mediated NF-kappaB or apoptosis pathways, and identified as an antiapoptotic receptor, although it retains one third of a consensus death domain motif in its cytoplasmic domain. It has been demonstrated that TRAILR4 expression is directly regulated by p53 and attenuates cell response to DNA-damaging agents.
