Human TNFRII Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10417-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_001066.2

cDNA Size:   1386

Synonym:  p75, TBPII, TNFBR, TNFR2, CD120b, TNFR1B, TNFR80, TNF-R75, p75TNFR, TNF-R-II, TNFRSF1B

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 168 A/G not causing the amino acid variation, and 587T/G resulting in the amino acid 196Met substitution by Arg.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGCGCCCG TCGCCGTCTG GGCCGCGCTG GCCGTCGGAC TGGAGCTCTG GGCTGCGGCG
61       CACGCCTTGC CCGCCCAGGT GGCATTTACA CCCTACGCCC CGGAGCCCGG GAGCACATGC
121      CGGCTCAGAG AATACTATGA CCAGACAGCT CAGATGTGCT GCAGCAAGTG CTCGCCGGGC
181      CAACATGCAA AAGTCTTCTG TACCAAGACC TCGGACACCG TGTGTGACTC CTGTGAGGAC
241      AGCACATACA CCCAGCTCTG GAACTGGGTT CCCGAGTGCT TGAGCTGTGG CTCCCGCTGT
301      AGCTCTGACC AGGTGGAAAC TCAAGCCTGC ACTCGGGAAC AGAACCGCAT CTGCACCTGC
361      AGGCCCGGCT GGTACTGCGC GCTGAGCAAG CAGGAGGGGT GCCGGCTGTG CGCGCCGCTG
421      CGCAAGTGCC GCCCGGGCTT CGGCGTGGCC AGACCAGGAA CTGAAACATC AGACGTGGTG
481      TGCAAGCCCT GTGCCCCGGG GACGTTCTCC AACACGACTT CATCCACGGA TATTTGCAGG
541      CCCCACCAGA TCTGTAACGT GGTGGCCATC CCTGGGAATG CAAGCAGGGA TGCAGTCTGC
601      ACGTCCACGT CCCCCACCCG GAGTATGGCC CCAGGGGCAG TACACTTACC CCAGCCAGTG
661      TCCACACGAT CCCAACACAC GCAGCCAACT CCAGAACCCA GCACTGCTCC AAGCACCTCC
721      TTCCTGCTCC CAATGGGCCC CAGCCCCCCA GCTGAAGGGA GCACTGGCGA CTTCGCTCTT
781      CCAGTTGGAC TGATTGTGGG TGTGACAGCC TTGGGTCTAC TAATAATAGG AGTGGTGAAC
841      TGTGTCATCA TGACCCAGGT GAAAAAGAAG CCCTTGTGCC TGCAGAGAGA AGCCAAGGTG
901      CCTCACTTGC CTGCCGATAA GGCCCGGGGT ACACAGGGCC CCGAGCAGCA GCACCTGCTG
961      ATCACAGCGC CGAGCTCCAG CAGCAGCTCC CTGGAGAGCT CGGCCAGTGC GTTGGACAGA
1021     AGGGCGCCCA CTCGGAACCA GCCACAGGCA CCAGGCGTGG AGGCCAGTGG GGCCGGGGAG
1081     GCCCGGGCCA GCACCGGGAG CTCAGATTCT TCCCCTGGTG GCCATGGGAC CCAGGTCAAT
1141     GTCACCTGCA TCGTGAACGT CTGTAGCAGC TCTGACCACA GCTCACAGTG CTCCTCCCAA
1201     GCCAGCTCCA CAATGGGAGA CACAGATTCC AGCCCCTCGG AGTCCCCGAA GGACGAGCAG
1261     GTCCCCTTCT CCAAGGAGGA ATGTGCCTTT CGGTCACAGC TGGAGACGCC AGAGACCCTG
1321     CTGGGGAGCA CCGAAGAGAA GCCCCTGCCC CTTGGAGTGC CTGATGCTGG GATGAAGCCC
1381     AGTTAA

Quality Control: cDNA sequencing results is available upon request.

TNFR2/CD120b/TNFRSF1B related areas, pathways, and other information

Cytokine