TNFR1 / CD120a / TNFRSF1A Antibody (Cytokine)

TNFR1 / CD120a / TNFRSF1A Antibody Datasheet

TNFR1 / CD120a / TNFRSF1A Antibody Product Information

• Immunogen: Recombinant Mouse TNFR1 / CD120a / TNFRSF1A protein (Catalog#50496-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 028
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFR1 / CD120a / TNFRSF1A (rM TNFR1 / CD120a / TNFRSF1A; Catalog#50496-M08H; NP_035739.2; Met 1-Ala 212).

TNFR1 / CD120a / TNFRSF1A Antibody Usage Guide

• Specificity: Mouse TNFR1 / CD120a / TNFRSF1A
• No cross-reactivity in ELISA with

  Human TNFRII

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse TNFRSF1A. The detection limit for Mouse TNFRSF1A is approximately 0. 00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

TNFR1 / CD120a / TNFRSF1A Antibody Background

Tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A), also known as TNFRI, TNF R-p55/p60, and CD120a, is a type I transmembrane protein widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. Human TNFRSF1A/TNFRI is a 455 aa protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain which mediates constitutive trimer formation followed by four TNF-α trimer-binding cysteine-rich domains (CRDs), a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain. TNFRSF1A/TNFRI serves as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor, and the resulting death-inducing signaling complex (DISC) initiates the subsequent cascade of caspases mediating apoptosis. TNFRSF1A/TNFRI can also activate the transcription factor NF-kB, and functions as a regulator of inflammation. Germline mutations of the extracellular domains of TNF RI are found to be associated with the human genetic disorder called periodic fever syndrome.

References

1. Lewis, M. et al., 1991, Proc. Natl. Acad. Sci. USA. 88: 2830-2834.
2. Banner, D.W. et al., 1993, Cell.73: 431-445.
3. Medvedev, A.E. et al., 1996, J. Biol. Chem. 271: 9778-9784.
4. Peschon, J.J. et al., 1998, J. Immunol. 160: 943-952.
5. Chan, F.K. et al., 2000, Science. 288: 2351-2354.
6. Micheau, O. et al., 2003, Cell. 114 (2): 181-190.
7. Hehlgans, T. et al., 2005, Immunology. 115: 1-20.