|Recombinant Mouse TNFR1 / CD120a / TNFRSF1A protein (Catalog#50496-M08H)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFR1 / CD120a / TNFRSF1A (rM TNFR1 / CD120a / TNFRSF1A; Catalog#50496-M08H; NP_035739.2; Met 1-Ala 212).|
No cross-reactivity in ELISA with
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse TNFRSF1A. The detection limit for Mouse TNFRSF1A is approximately 0. 00245 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Mouse TNFR1 / TNFRSF1A / CD120a in ELISA pair set (Catalog: # SEK50496). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50496-R104).
Application: WB IHC-P
Application: ELISA WB
Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution
Lane A: NIH3T3 Whole Cell Lysate
Lane B: Hela Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:15 kDa
Observed band size:17 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.