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Your Position: Home > Gene Products > Other Species > Ferret TNFA / TNF-alpha Gene cDNA Clone / ORF Clone

Ferret TNFA / TNF-alpha Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
FG60002-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

TNFA / TNF-alpha cDNA Clone Datasheet

TNFA / TNF-alpha cDNA Clone Product Information

Gene Bank Ref.ID:	EF368211.1
cDNA Size:	702
Gene Synonym:	TNFA
Source:	Ferret

TNFA / TNF-alpha cDNA Clone Description

cDNA Description:	ORF Clone of Ferret TNF alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 27 C/T and 480 T/C not causing the amino acid variation.

TNFA / TNF-alpha cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFA / TNF-alpha cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TNFA / TNF-alpha cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCACTG AAAGCATGAT CCGGGATGTG GAGCTGGGCG AGGAGGCACT CCCCAAGAAG
61       GCAGGGGCCC CCAAGGGCTC CCGAAGGTGC TGGTGCCTCA GCCTCTTCTC CTTCCTCCTC
121      GTCGCAGGAG CCACCACACT GTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
181      GAAGAGCTGC CAGATGGCCT CCAACTAATC AAACCTCTGG CCCAGACAGT CAAATCATCT
241      TCTCGAACTC CAAGTGACAA GCCTGTAGCT CATGTTGTAG CAAACCCTGA AGCTGAGGGG
301      CAACTCCAAT GGCTGAGCCG ACGTGCCAAT GCCCTCCTGG CCAATGGTGT GGAGCTGACA
361      GACAACCAGC TAATAGTGCC ATCAGACGGG CTGTACCTTA TCTACTCGCA GGTCCTCTTC
421      AAGGGCCGAG GATGTTCTTC CACCAATGTG CTCCTTACCC ACACCATCAG CCGCTTCGCC
481      GTCTCCTACC AGACCAAGGT CAACCTCCTG TCTGCCATCA AGAGCCCTTG CCAAAGGGAA
541      ACCCCAGAGG GGACTGAGGC CAAGCCCTGG TACGAGCCCA TCTACCTGGG AGGGGTCTTC
601      CAACTGGAGA AGGGGGATCG ACTCAGCGCT GAGATCAACC TGCCTGCCTA TCTCGACTTT
661      GCCGAATCCG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

Quality Control:cDNA sequencing results is available upon request.

Cytokine